JBO Letters

Robust overlay schemes for the fusion of fluorescence and color channels in biological imaging

[+] Author Affiliations
Jürgen Glatz, P. Beatriz Garcia-Allende

Technische Universität München, Chair for Biological Imaging, Trogerstr. 9, D-81675 Munich, Germany

Panagiotis Symvoulidis

Institute for Biological and Medical Imaging, Helmholtz Zentrum München, Ingolstädter Landstr. 1, D-85764 Neuherberg, Germany

Vasilis Ntziachristos

Technische Universität München, Chair for Biological Imaging, Trogerstr. 9, D-81675 Munich, Germany

Institute for Biological and Medical Imaging, Helmholtz Zentrum München, Ingolstädter Landstr. 1, D-85764 Neuherberg, Germany

J. Biomed. Opt. 19(4), 040501 (Apr 02, 2014). doi:10.1117/1.JBO.19.4.040501
History: Received January 24, 2014; Revised February 19, 2014; Accepted February 27, 2014
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Abstract.  Molecular fluorescence imaging is a commonly used method in various biomedical fields and is undergoing rapid translation toward clinical applications. Color images are commonly superimposed with fluorescence measurements to provide orientation, anatomical information, and molecular tissue properties in a single image. New adaptive methods that produce a more robust composite image than conventional lime green alpha blending are presented and demonstrated herein. Moreover, visualization through temporal changes is showcased as an alternative for real-time imaging systems.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Topics

Luminescence

Citation

Jürgen Glatz ; Panagiotis Symvoulidis ; P. Beatriz Garcia-Allende and Vasilis Ntziachristos
"Robust overlay schemes for the fusion of fluorescence and color channels in biological imaging", J. Biomed. Opt. 19(4), 040501 (Apr 02, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.4.040501


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