Research Papers: Sensing

Real-time measurements of endogenous carbon monoxide production in isolated pig lungs

[+] Author Affiliations
Maxime Maignan, Raphael Briot, Stephane Gennai, Guillaume Debaty

Centre Hospitalier Universitaire Michallon, Emergency Department and Mobile Intensive Care Unit, 38043 Grenoble Cedex 09, France

Université Joseph Fourier Grenoble 1, /CNRS/TIMC-IMAG UMR 5525/PRETA Team, Grenoble F-38041, France

Daniel Romanini, Irene Ventrillard

Université Grenoble 1/CNRS, LiPhy UMR 5588, Grenoble F-38041, France

Florence Hazane-Puch

Centre Hospitalier Universitaire de Grenoble, Institut de Biologie et de Pathologie, Département de Biochimie, Toxicologie et Pharmacologie, Unité de Biochimie Hormonale et Nutritionnelle, CS 10217, 38043 Grenoble, France

Angelique Brouta

Université Joseph Fourier Grenoble 1, /CNRS/TIMC-IMAG UMR 5525/PRETA Team, Grenoble F-38041, France

J. Biomed. Opt. 19(4), 047001 (Apr 03, 2014). doi:10.1117/1.JBO.19.4.047001
History: Received June 24, 2013; Revised February 12, 2014; Accepted March 10, 2014
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Abstract.  Ischemia-reperfusion injuries are a critical determinant of lung transplantation success. The endogenous production of carbon monoxide (CO) is triggered by ischemia-reperfusion injuries. Our aim was, therefore, to assess the feasibility of exhaled CO measurements during the ex vivo evaluation of lungs submitted to ischemia-reperfusion injuries. Five pigs were euthanized and their lungs removed after pneumoplegia. After cold storage (30 min, 4°C), the lungs were connected to an extracorporeal membrane oxygenation circuit, slowly warmed-up, and ventilated. At the end of a 45-min steady state, CO measurements were performed by optical-feedback cavity-enhanced absorption spectroscopy, a specific laser-based technique for noninvasive and real-time low gas concentration measurements. Exhaled CO concentration from isolated lungs reached 0.45±0.19ppmv and was above CO concentration in ambient air and in medical gas. CO variations peaked during the expiratory phase. Changes in CO concentration in ambient air did not alter CO concentrations in isolated lungs. Exhaled CO level was also found to be uncorrelated to heme oxygenase (HO-1) gene expression. These results confirm the feasibility of accurate and real-time CO measurement in isolated lungs. The presented technology could help establishing the exhaled CO concentration as a biomarker of ischemia-reperfusion injury in ex vivo lung perfusion.

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Citation

Maxime Maignan ; Raphael Briot ; Daniel Romanini ; Stephane Gennai ; Florence Hazane-Puch, et al.
"Real-time measurements of endogenous carbon monoxide production in isolated pig lungs", J. Biomed. Opt. 19(4), 047001 (Apr 03, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.4.047001


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