Research Papers: General

Four-dimensional motility tracking of biological cells by digital holographic microscopy

[+] Author Affiliations
Xiao Yu

University of South Florida, Department of Physics, Digital Holography and Microscopy Laboratory, Tampa, Florida 33620

Jisoo Hong

University of South Florida, Department of Physics, Digital Holography and Microscopy Laboratory, Tampa, Florida 33620

Changgeng Liu

University of South Florida, Department of Physics, Digital Holography and Microscopy Laboratory, Tampa, Florida 33620

Michael Cross

University of South Florida, Department of Physics, Nanomedicine and Nanobiotechnology Laboratory, Tampa, Florida 33620

Donald T. Haynie

University of South Florida, Department of Physics, Nanomedicine and Nanobiotechnology Laboratory, Tampa, Florida 33620

Myung K. Kim

University of South Florida, Department of Physics, Digital Holography and Microscopy Laboratory, Tampa, Florida 33620

J. Biomed. Opt. 19(4), 045001 (Apr 03, 2014). doi:10.1117/1.JBO.19.4.045001
History: Received January 15, 2014; Revised March 2, 2014; Accepted March 10, 2014
Text Size: A A A

Abstract.  Three-dimensional profiling and tracking by digital holography microscopy (DHM) provide label-free and quantitative analysis of the characteristics and dynamic processes of objects, since DHM can record real-time data for microscale objects and produce a single hologram containing all the information about their three-dimensional structures. Here, we have utilized DHM to visualize suspended microspheres and microfibers in three dimensions, and record the four-dimensional trajectories of free-swimming cells in the absence of mechanical focus adjustment. The displacement of microfibers due to interactions with cells in three spatial dimensions has been measured as a function of time at subsecond and micrometer levels in a direct and straightforward manner. It has thus been shown that DHM is a highly efficient and versatile means for quantitative tracking and analysis of cell motility.

Figures in this Article
© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Xiao Yu ; Jisoo Hong ; Changgeng Liu ; Michael Cross ; Donald T. Haynie, et al.
"Four-dimensional motility tracking of biological cells by digital holographic microscopy", J. Biomed. Opt. 19(4), 045001 (Apr 03, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.4.045001


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.