Research Papers: Imaging

Label-free cell-based assay with spectral-domain optical coherence phase microscopy

[+] Author Affiliations
Suho Ryu, Kyung-A Hyun, Jung Heo, Hyo-Il Jung, Chulmin Joo

Yonsei University, School of Mechanical Engineering, Seoul 120-749, Republic of Korea

J. Biomed. Opt. 19(4), 046003 (Apr 07, 2014). doi:10.1117/1.JBO.19.4.046003
History: Received December 3, 2013; Revised February 18, 2014; Accepted March 10, 2014
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Abstract.  Quantitative measurement of dynamic responses of unstained living cells is of great importance in many applications ranging from investigation of fundamental cellular functions to drug discoveries. Conventional optical methods for label-free cell-based assay examine cellular structural changes proximal to sensor surfaces under external stimuli, but require dedicated nanostructure-patterned substrates for operation. Here, we present a quantitative imaging method, spectral-domain optical coherence phase microscopy (SD-OCPM), as a viable optical platform for label-free cell-based assay. The instrument is based on a low-coherence interferometric microscope that enables quantitative depth-resolved phase measurement of a transparent specimen with high phase stability. We demonstrate SD-OCPM measurement of dynamic responses of human breast cancer cells (MCF-7) to 2-picolinic acid (PA) and histamine.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Suho Ryu ; Kyung-A Hyun ; Jung Heo ; Hyo-Il Jung and Chulmin Joo
"Label-free cell-based assay with spectral-domain optical coherence phase microscopy", J. Biomed. Opt. 19(4), 046003 (Apr 07, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.4.046003


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