Research Papers: Imaging

Calibration-free structured-illumination photoacoustic flowgraphy of transverse flow in scattering media

[+] Author Affiliations
Junjie Yao

Washington University in St. Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, St. Louis, Missouri 63130

Rebecca C. Gilson

Washington University in St. Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, St. Louis, Missouri 63130

Konstantin I. Maslov

Washington University in St. Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, St. Louis, Missouri 63130

Lidai Wang

Washington University in St. Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, St. Louis, Missouri 63130

Lihong V. Wang

Washington University in St. Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, St. Louis, Missouri 63130

J. Biomed. Opt. 19(4), 046007 (Apr 09, 2014). doi:10.1117/1.JBO.19.4.046007
History: Received December 5, 2013; Revised February 27, 2014; Accepted March 21, 2014
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Abstract.  We propose a calibration-free photoacoustic (PA) method for transverse flow measurements. In this method, a pulsed periodically structured (i.e., grating patterned) optical beam is used to illuminate flowing absorptive particles in an optically scattering medium. The PA signal amplitudes measured over consecutive laser pulses carry an imprint of the illumination structure. The modulation frequency of the imprint is proportional to the component of the flow speed projected onto the normal axis of the striped illumination pattern. This method can tolerate high particle density, and is insensitive to the particle size, thus calibration-free. Bovine blood and microsphere phantoms were used to validate the proposed method. Blood flow in a mouse ear was measured in vivo as well.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Junjie Yao ; Rebecca C. Gilson ; Konstantin I. Maslov ; Lidai Wang and Lihong V. Wang
"Calibration-free structured-illumination photoacoustic flowgraphy of transverse flow in scattering media", J. Biomed. Opt. 19(4), 046007 (Apr 09, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.4.046007


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