Research Papers: Imaging

Tomographic phase microscopy of living three-dimensional cell cultures

[+] Author Affiliations
Arkadiusz Kuś

Warsaw University of Technology, Institute of Micromechanics and Photonics, Photonics Engineering Division, Faculty of Mechatronics, Św. A. Boboli 8 Street, 02-525 Warsaw, Poland

Michał Dudek

Warsaw University of Technology, Institute of Micromechanics and Photonics, Photonics Engineering Division, Faculty of Mechatronics, Św. A. Boboli 8 Street, 02-525 Warsaw, Poland

Björn Kemper

University of Münster, Center for Biomedical Optics and Photonics, Robert-Koch-Strasse 45, 48149 Münster, Germany

University of Münster, Biomedical Technology Center of the Medical Faculty, Mendelstraße 17 17, 48149 Münster, Germany

Małgorzata Kujawińska

Warsaw University of Technology, Institute of Micromechanics and Photonics, Photonics Engineering Division, Faculty of Mechatronics, Św. A. Boboli 8 Street, 02-525 Warsaw, Poland

Angelika Vollmer

University of Münster, Center for Biomedical Optics and Photonics, Robert-Koch-Strasse 45, 48149 Münster, Germany

J. Biomed. Opt. 19(4), 046009 (Apr 10, 2014). doi:10.1117/1.JBO.19.4.046009
History: Received November 14, 2013; Revised February 25, 2014; Accepted March 17, 2014
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Abstract.  A successful application of self-interference digital holographic microscopy in combination with a sample-rotation-based tomography module for three-dimensional (3-D) label-free quantitative live cell imaging with subcellular resolution is demonstrated. By means of implementation of a hollow optical fiber as the sample cuvette, the observation of living cells in different 3-D matrices is enabled. The fiber delivers a stable and accurate rotation of a cell or cell cluster, providing quantitative phase data for tomographic reconstruction of the 3-D refractive index distribution with an isotropic spatial resolution. We demonstrate that it is possible to clearly distinguish and quantitatively analyze several cells grouped in a “3-D cluster” as well as subcellular organelles like the nucleoli and local internal refractive index changes.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Arkadiusz Kuś ; Michał Dudek ; Björn Kemper ; Małgorzata Kujawińska and Angelika Vollmer
"Tomographic phase microscopy of living three-dimensional cell cultures", J. Biomed. Opt. 19(4), 046009 (Apr 10, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.4.046009


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