Research Papers: General

High-purity separation of cancer cells by optically induced dielectrophoresis

[+] Author Affiliations
Hsiu-Hsiang Chen

Industrial Technology Research Institute, P.O. Box 31054, Room 308, Building 78, 195, Section 4, Chung Hsing Road, Chutung, Hsinchu, Taiwan

Mai-Wei Lin

Industrial Technology Research Institute, P.O. Box 31054, Room 308, Building 78, 195, Section 4, Chung Hsing Road, Chutung, Hsinchu, Taiwan

Wan-Ting Tien

Industrial Technology Research Institute, P.O. Box 31054, Room 308, Building 78, 195, Section 4, Chung Hsing Road, Chutung, Hsinchu, Taiwan

Chin-Pen Lai

Industrial Technology Research Institute, P.O. Box 31054, Room 308, Building 78, 195, Section 4, Chung Hsing Road, Chutung, Hsinchu, Taiwan

Kuo-Yao Weng

Industrial Technology Research Institute, P.O. Box 31054, Room 308, Building 78, 195, Section 4, Chung Hsing Road, Chutung, Hsinchu, Taiwan

Ching-Huai Ko

Industrial Technology Research Institute, P.O. Box 31054, Room 308, Building 78, 195, Section 4, Chung Hsing Road, Chutung, Hsinchu, Taiwan

Chun-Chuan Lin

Industrial Technology Research Institute, P.O. Box 31054, Room 308, Building 78, 195, Section 4, Chung Hsing Road, Chutung, Hsinchu, Taiwan

Jyh-Chern Chen

Industrial Technology Research Institute, P.O. Box 31054, Room 308, Building 78, 195, Section 4, Chung Hsing Road, Chutung, Hsinchu, Taiwan

Kuo-Tung Tiao

Industrial Technology Research Institute, P.O. Box 31054, Room 308, Building 78, 195, Section 4, Chung Hsing Road, Chutung, Hsinchu, Taiwan

Tse-Ching Chen

Chang Gung University, Chang Gung Memorial Hospital, P.O. Box 33305, 259 Wen-Hwa 1st Road, Kwei-Shan Tao-Yuan, Taiwan

Shin-Cheh Chen

Chang Gung University, Chang Gung Memorial Hospital, P.O. Box 33305, 259 Wen-Hwa 1st Road, Kwei-Shan Tao-Yuan, Taiwan

Ta-Sen Yeh

Chang Gung University, Chang Gung Memorial Hospital, P.O. Box 33305, 259 Wen-Hwa 1st Road, Kwei-Shan Tao-Yuan, Taiwan

Chieh-Fang Cheng

Industrial Technology Research Institute, P.O. Box 31054, Room 308, Building 78, 195, Section 4, Chung Hsing Road, Chutung, Hsinchu, Taiwan

J. Biomed. Opt. 19(4), 045002 (Apr 10, 2014). doi:10.1117/1.JBO.19.4.045002
History: Received December 26, 2013; Revised March 12, 2014; Accepted March 14, 2014
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Abstract.  Detecting and concentrating cancer cells in peripheral blood is of great importance for cancer diagnosis and prognosis. Optically induced dielectrophoresis (ODEP) can achieve high resolution and low optical intensities, and the electrode pattern can be dynamically changed by varied light patterns. By changing the projected light pattern, it is demonstrated to separate high-purity gastric cancer cell lines. Traditionally, the purity of cancer cell isolation by negative selection is 0.9% to 10%; by positive selection it is 50% to 62%. An ODEP technology is proposed to enhance the purity of cancer cell isolation to about 77%.

© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Hsiu-Hsiang Chen ; Mai-Wei Lin ; Wan-Ting Tien ; Chin-Pen Lai ; Kuo-Yao Weng, et al.
"High-purity separation of cancer cells by optically induced dielectrophoresis", J. Biomed. Opt. 19(4), 045002 (Apr 10, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.4.045002


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