Research Papers: Imaging

Video-rate optical flow corrected intraoperative functional fluorescence imaging

[+] Author Affiliations
Maximilian Koch, Jürgen Glatz, Vladimir Ermolayev, Vasilis Ntziachristos

Technische Universität München, Institute for Biological and Medical Imaging, & Helmholtz Zentrum München, German Research Center for Environment and Health (GmbH), Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany

Elisabeth G. E. de Vries

University Medical Centre Groningen, Medical Oncology , 9700 RB Groningen, The Netherlands

Gooitzen M. van Dam

University Medical Centre Groningen, Department of Surgery, 9700 RB Groningen, The Netherlands

Karl-Hans Englmeier

Technische Universität München, Institute for Biological and Medical Imaging, & Helmholtz Zentrum München, German Research Center for Environment and Health (GmbH), Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany

Helmholtz Zentrum München, German Research Center for Environment and Health (GmbH), Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany

J. Biomed. Opt. 19(4), 046012 (Apr 21, 2014). doi:10.1117/1.JBO.19.4.046012
History: Received December 13, 2013; Revised March 7, 2014; Accepted March 17, 2014
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Abstract.  Intraoperative fluorescence molecular imaging based on targeted fluorescence agents is an emerging approach to improve surgical and endoscopic imaging and guidance. Short exposure times per frame and implementation at video rates are necessary to provide continuous feedback to the physician and avoid motion artifacts. However, fast imaging implementations also limit the sensitivity of fluorescence detection. To improve on detection sensitivity in video rate fluorescence imaging, we considered herein an optical flow technique applied to texture-rich color images. This allows the effective accumulation of fluorescence signals over longer, virtual exposure times. The proposed correction scheme is shown to improve signal-to-noise ratios both in phantom experiments and in vivo tissue imaging.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Maximilian Koch ; Jürgen Glatz ; Vladimir Ermolayev ; Elisabeth G. E. de Vries ; Gooitzen M. van Dam, et al.
"Video-rate optical flow corrected intraoperative functional fluorescence imaging", J. Biomed. Opt. 19(4), 046012 (Apr 21, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.4.046012


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