Research Papers: Imaging

Second harmonic generation polarization properties of myofilaments

[+] Author Affiliations
Masood Samim, Nicole Prent, Virginijus Barzda

University of Toronto, Department of Physics and Institute for Optical Sciences, 60 Saint George Street, Toronto, Ontario M5S1A7, Canada

University of Toronto Mississauga, Department of Chemical and Physical Sciences, 3359 Mississauga Road North, Mississauga, Ontario L5L1C6, Canada

Daniel Dicenzo

University of Toronto Mississauga, Department of Chemical and Physical Sciences, 3359 Mississauga Road North, Mississauga, Ontario L5L1C6, Canada

Bryan Stewart

University of Toronto Mississauga, Department of Biology, 3359 Mississauga Road North, Mississauga, Ontario L5L1C6, Canada

J. Biomed. Opt. 19(5), 056005 (May 07, 2014). doi:10.1117/1.JBO.19.5.056005
History: Received February 13, 2014; Revised March 24, 2014; Accepted April 11, 2014
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Abstract.  Second harmonic generation (SHG) polarization microscopy was used to investigate the organization of myosin nanomotors in myofilaments of muscle cells. The distribution of the second-order nonlinear susceptibility component ratio χzzz(2)/χzxx(2) along anisotropic bands of sarcomeres revealed differences between the headless and head-containing regions of myofilaments. The polarization-in polarization-out SHG measurements of headless myosin mutants of indirect flight muscle in Drosophila melanogaster confirmed a lower susceptibility component ratio compared to the head-containing myocytes with wild-type myosins. The increase in the ratio is assigned to the change in the deflection angle of the myosin S2 domain and possible contribution of myosin heads. The nonlinear susceptibility component ratio is a sensitive indicator of the myosin structure, and therefore, it can be used for conformational studies of myosin nanomotors. The measured ratio values can also be used as the reference for ab initio calculations of nonlinear optical properties of different parts of myosins.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Masood Samim ; Nicole Prent ; Daniel Dicenzo ; Bryan Stewart and Virginijus Barzda
"Second harmonic generation polarization properties of myofilaments", J. Biomed. Opt. 19(5), 056005 (May 07, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.5.056005


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