Research Papers: Sensing

Monitoring of the action of drugs in melanoma cells by dynamic laser speckle

[+] Author Affiliations
Rolando J. González-Peña

Universidad de Valencia, Unidad de Biofísica y Física Médica, Departamento de Fisiología, Facultad de Medicina y Odontología, Valencia, Spain, CP 46010

Roberto A. Braga, Jr.

Universidade Federal de Lavras, Departamento de Engenharia, LavrasMG, Brazil, CP 3037

Rosa M. Cibrián

Universidad de Valencia, Unidad de Biofísica y Física Médica, Departamento de Fisiología, Facultad de Medicina y Odontología, Valencia, Spain, CP 46010

Rosario Salvador-Palmer

Universidad de Valencia, Unidad de Biofísica y Física Médica, Departamento de Fisiología, Facultad de Medicina y Odontología, Valencia, Spain, CP 46010

Rosario Gil-Benso

Universidad de Valencia, Departamento de Patología, Facultad de Medicina y Odontología, Valencia, Spain, CP 46010

Teresa San Miguel

Universidad de Valencia, Departamento de Patología, Facultad de Medicina y Odontología, Valencia, Spain, CP 46010

J. Biomed. Opt. 19(5), 057008 (May 14, 2014). doi:10.1117/1.JBO.19.5.057008
History: Received January 24, 2014; Revised April 3, 2014; Accepted April 23, 2014
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Abstract.  This work presents the development of a protocol based on the dynamic laser speckle designed to monitor the reaction of cancer cells of line MEL-RC08 to the application of the drug Colcemid in two different concentrations: 0.2 and 0.4μg/mL. The protocol was designed using the forward scattering approach with an He-Ne laser of 632.8 nm illuminating the samples, a control, and two variations of Colcemid, being monitored along 8 h. The data were analyzed numerically in the time and in the frequency domain, and the results presented the ability of the technique to monitor the action of the drug, particularly Colcemid (0.4μg/mL).

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Rolando J. González-Peña ; Roberto A. Braga, Jr. ; Rosa M. Cibrián ; Rosario Salvador-Palmer ; Rosario Gil-Benso, et al.
"Monitoring of the action of drugs in melanoma cells by dynamic laser speckle", J. Biomed. Opt. 19(5), 057008 (May 14, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.5.057008


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