Mice were imaged using the Olympus OV100 (Olympus Corp., Tokyo, Japan), containing an MT-20 light source (Olympus Biosystems, Planegg, Germany), and DP70 CCD camera (Olympus Corp., Tokyo, Japan). The OV100 was used due to its unique ability to accomplish high fidelity fluorescence imaging with variable magnification capabilities that allow for imaging of not only the whole animal, but also at the subcellular level. The instrument incorporates a unique combination of a high numerical aperture and long working distance. Four individually optimized objective lenses, parcentered, and parfocal provide a fold magnification range for seamless imaging of the entire body down to the subcellular level without disturbing the animal.8 In addition, openings on the machine allow for delivery of inhalational anesthesia to the animal without allowing entry of outside light. This allows for capturing high quality in vivo images with minimal morbidity to the animal by avoiding anesthetic injections. All images were analyzed using Image-J (National Institute of Health, Bethesda, Maryland) and were processed with the use of Photoshop Elements 11 (Adobe Systems Inc., San Jose, California).