Research Papers: Imaging

Rotational multispectral fluorescence lifetime imaging and intravascular ultrasound: bimodal system for intravascular applications

[+] Author Affiliations
Dinglong Ma

University of California, Department of Biomedical Engineering, 451 Health Sciences Drive, Davis, California 95616

Julien Bec

University of California, Department of Biomedical Engineering, 451 Health Sciences Drive, Davis, California 95616

Diego R. Yankelevich

University of California, Department of Biomedical Engineering, 451 Health Sciences Drive, Davis, California 95616

University of California, Department of Electrical Engineering, 3101 Kemper Hall, Davis, California 95616

Dimitris Gorpas

University of California, Department of Biomedical Engineering, 451 Health Sciences Drive, Davis, California 95616

Hussain Fatakdawala

University of California, Department of Biomedical Engineering, 451 Health Sciences Drive, Davis, California 95616

Laura Marcu

University of California, Department of Biomedical Engineering, 451 Health Sciences Drive, Davis, California 95616

J. Biomed. Opt. 19(6), 066004 (Jun 04, 2014). doi:10.1117/1.JBO.19.6.066004
History: Received February 17, 2014; Revised May 5, 2014; Accepted May 6, 2014
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Abstract.  We report the development and validation of a hybrid intravascular diagnostic system combining multispectral fluorescence lifetime imaging (FLIm) and intravascular ultrasound (IVUS) for cardiovascular imaging applications. A prototype FLIm system based on fluorescence pulse sampling technique providing information on artery biochemical composition was integrated with a commercial IVUS system providing information on artery morphology. A customized 3-Fr bimodal catheter combining a rotational side-view fiberoptic and a 40-MHz IVUS transducer was constructed for sequential helical scanning (rotation and pullback) of tubular structures. Validation of this bimodal approach was conducted in pig heart coronary arteries. Spatial resolution, fluorescence detection efficiency, pulse broadening effect, and lifetime measurement variability of the FLIm system were systematically evaluated. Current results show that this system is capable of temporarily resolving the fluorescence emission simultaneously in multiple spectral channels in a single pullback sequence. Accurate measurements of fluorescence decay characteristics from arterial segments can be obtained rapidly (e.g., 20 mm in 5 s), and accurate co-registration of fluorescence and ultrasound features can be achieved. The current finding demonstrates the compatibility of FLIm instrumentation with in vivo clinical investigations and its potential to complement conventional IVUS during catheterization procedures.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Dinglong Ma ; Julien Bec ; Diego R. Yankelevich ; Dimitris Gorpas ; Hussain Fatakdawala, et al.
"Rotational multispectral fluorescence lifetime imaging and intravascular ultrasound: bimodal system for intravascular applications", J. Biomed. Opt. 19(6), 066004 (Jun 04, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.6.066004


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