Special Section on Biomimetic and Bioinspired Materials for Applications in Biophotonics

Quantitative evaluation and visualization of size effect on cellular uptake of gold nanoparticles by multiphoton imaging-UV/Vis spectroscopic analysis

[+] Author Affiliations
Ke Li

Huazhong University of Science and Technology, Pharmacy Department, Union Hospital, Tongji Medical College, Wuhan 430022, China

Marc Schneider

Philipps University Marburg, Pharmaceutics and Biopharmacy, Ketzerbach 63, D-35037 Marburg, Germany

J. Biomed. Opt. 19(10), 101505 (Jun 13, 2014). doi:10.1117/1.JBO.19.10.101505
History: Received March 7, 2014; Revised May 16, 2014; Accepted May 19, 2014
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Abstract.  With ever-increasing applications of nanoscale materials in the biomedical field, the impact of nanoparticle size on cellular uptake efficiency, dynamics, and mechanism has attracted numerous interests but still leaves many open questions. A combined “multiphoton imaging-UV/Vis spectroscopic analysis” method was applied for the first time for quantitative visualization and evaluation of the cellular uptake process of different-sized (15-, 30-, 50-, and 80-nm) gold nanoparticles (AuNPs). Quantitative analysis of the size effect on cellular uptake behavior of AuNPs from a stack of three-dimensional multiphoton laser scanning microscopy images is obtained. The technique allows for differentiating AuNPs present in external and internal subcellular components, giving detailed information for elucidating cellular uptake dynamics without particle labeling. The data show that the internalization extent of AuNPs is highly dependent on particles’ sizes and incubation time. Due to sedimentation, 50- and 80-nm AuNPs are taken up to a greater extent than 15- and 30-nm particles after exposure for 24 h. However, the smaller particles’ uptake velocity is significantly faster in the first 10 h, indicating a disparity in uptake kinetics for different-sized AuNPs. The finding from this study will improve our understanding of the cellular uptake mechanisms of different-sized nanoparticles and has great implications in developing AuNP-based drug carriers with various sizes for different purposes.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Ke Li and Marc Schneider
"Quantitative evaluation and visualization of size effect on cellular uptake of gold nanoparticles by multiphoton imaging-UV/Vis spectroscopic analysis", J. Biomed. Opt. 19(10), 101505 (Jun 13, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.10.101505


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