Research Papers: Imaging

Identification of dirty necrosis in colorectal carcinoma based on multiphoton microscopy

[+] Author Affiliations
Lianhuang Li, Hongsheng Li, Jianxin Chen

Fujian Normal University, Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fuzhou 350007, China

Weizhong Jiang, Zhifen Chen, Guoxian Guan

Fujian Medical University, The Affiliated Union Hospital, Department of Colorectal Surgery, Fuzhou 350001, China

Yinghong Yang, Changyin Feng

Fujian Medical University, The Affiliated Union Hospital, Department of Pathology, Fuzhou 350001, China

J. Biomed. Opt. 19(6), 066008 (Jun 26, 2014). doi:10.1117/1.JBO.19.6.066008
History: Received February 26, 2014; Revised May 21, 2014; Accepted June 2, 2014
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Abstract.  Dirty necrosis within glandular lumina is often considered as a characteristic of colorectal carcinomas (CRCs) that is a diagnostically useful feature of CRCs with DNA microsatellite instability (MSI). Multiphoton microscopy (MPM), which is based on the second-harmonic generation and two-photon excited fluorescence signals, was used to identify dirty necrosis. Our results demonstrated that MPM has the ability to exhibit the microstructure of dirty necrosis and the signal intensity as well as an emission spectrum that can help to differentiate dirty necrosis from cancer cells. These findings indicate that MPM may be helpful in distinguishing MSI colorectal carcinoma via the identification of dirty necrosis.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Lianhuang Li ; Weizhong Jiang ; Yinghong Yang ; Zhifen Chen ; Changyin Feng, et al.
"Identification of dirty necrosis in colorectal carcinoma based on multiphoton microscopy", J. Biomed. Opt. 19(6), 066008 (Jun 26, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.6.066008


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