Special Section on Vibrational Spectroscopy and Imaging

Surface plasmon resonance–based infrared biosensor for cell studies with simultaneous control

[+] Author Affiliations
Alexander Zilbershtein

Hebrew University of Jerusalem, The Racah Institute of Physics, 91904 Jerusalem, Israel

Amir Bein

Hebrew University of Jerusalem, School of Nutritional Sciences, Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, P.O. Box 12, 76100 Rehovot, Israel

Vladislav Lirtsman

Hebrew University of Jerusalem, The Racah Institute of Physics, 91904 Jerusalem, Israel

Betty Schwartz

Hebrew University of Jerusalem, School of Nutritional Sciences, Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, P.O. Box 12, 76100 Rehovot, Israel

Michael Golosovsky

Hebrew University of Jerusalem, The Racah Institute of Physics, 91904 Jerusalem, Israel

Dan Davidov

Hebrew University of Jerusalem, The Racah Institute of Physics, 91904 Jerusalem, Israel

J. Biomed. Opt. 19(11), 111608 (Jun 27, 2014). doi:10.1117/1.JBO.19.11.111608
History: Received January 31, 2014; Revised May 13, 2014; Accepted May 27, 2014
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Abstract.  We report a label-free infrared surface plasmon biosensor with a double-chamber flow cell for continuous monitoring of morphological changes in cell culture exposed to various stimuli. In this technique, the monolayer of cultured cells is divided into two halves by a barrier, allowing the treatment of one half while the other serves as control. We demonstrate the advantages of this setup in test experiments that track kinetics of the IEC-18 cell layer response to variations in extracellular Ca2+ concentration. The sensitivity of the presented method was found to be an order of magnitude higher compared to the single-chamber biosensor.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Alexander Zilbershtein ; Amir Bein ; Vladislav Lirtsman ; Betty Schwartz ; Michael Golosovsky, et al.
"Surface plasmon resonance–based infrared biosensor for cell studies with simultaneous control", J. Biomed. Opt. 19(11), 111608 (Jun 27, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.11.111608


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