Research Papers: Imaging

Interferometric time-stretch microscopy for ultrafast quantitative cellular and tissue imaging at 1 μm

[+] Author Affiliations
Andy K. S. Lau

University of Hong Kong, Faculty of Engineering, Department of Electrical and Electronic Engineering, Pokfulam Road, Hong Kong, China

Terence T. W. Wong, Antony C. S. Chan, Xiaoming Wei, Edmund Y. Lam, Kenneth K. Y. Wong, Kevin K. Tsia

University of Hong Kong, Faculty of Engineering, Department of Electrical and Electronic Engineering, Pokfulam Road, Hong Kong, China

Kenneth K. Y. Ho, Matthew T. H. Tang

University of Hong Kong, Faculty of Engineering, Department of Mechanical Engineering, Pokfulam Road, Hong Kong, China

Ho Cheung Shum

University of Hong Kong, Faculty of Engineering, Department of Mechanical Engineering, Pokfulam Road, Hong Kong, China

University of Hong Kong-Shenzhen Institute of Research and Innovation, Shenzhen Software Park, Shenzhen, China

J. Biomed. Opt. 19(7), 076001 (Jul 01, 2014). doi:10.1117/1.JBO.19.7.076001
History: Received January 31, 2014; Revised May 21, 2014; Accepted May 29, 2014
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Abstract.  Quantitative phase imaging (QPI) has been proven to be a powerful tool for label-free characterization of biological specimens. However, the imaging speed, largely limited by the image sensor technology, impedes its utility in applications where high-throughput screening and efficient big-data analysis are mandated. We here demonstrate interferometric time-stretch (iTS) microscopy for delivering ultrafast quantitative phase cellular and tissue imaging at an imaging line-scan rate >20MHz—orders-of-magnitude faster than conventional QPI. Enabling an efficient time-stretch operation in the 1-μm wavelength window, we present an iTS microscope system for practical ultrafast QPI of fixed cells and tissue sections, as well as ultrafast flowing cells (at a flow speed of up to 8m/s). To the best of our knowledge, this is the first time that time-stretch imaging could reveal quantitative morphological information of cells and tissues with nanometer precision. As many parameters can be further extracted from the phase and can serve as the intrinsic biomarkers for disease diagnosis, iTS microscopy could find its niche in high-throughput and high-content cellular assays (e.g., imaging flow cytometry) as well as tissue refractometric imaging (e.g., whole-slide imaging for digital pathology).

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Andy K. S. Lau ; Terence T. W. Wong ; Kenneth K. Y. Ho ; Matthew T. H. Tang ; Antony C. S. Chan, et al.
"Interferometric time-stretch microscopy for ultrafast quantitative cellular and tissue imaging at 1 μm", J. Biomed. Opt. 19(7), 076001 (Jul 01, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.7.076001


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