Special Section on Biomimetic and Bioinspired Materials for Applications in Biophotonics

Europium-quantum dot nanobioconjugates as luminescent probes for time-gated biosensing

[+] Author Affiliations
Piotr J. Cywiński

University of Potsdam, Institute of Chemistry, Physical Chemistry, Karl-Liebknecht-Str. 24-25, Potsdam-Golm 14476, Germany

Fraunhofer Institute for Applied Polymer Research, NanoPolyPhotonics, Geiselbergstr. 69, Potsdam-Golm 14476, Germany

Tommy Hammann

University of Potsdam, Institute of Chemistry, Physical Chemistry, Karl-Liebknecht-Str. 24-25, Potsdam-Golm 14476, Germany

Fraunhofer Institute for Applied Polymer Research, NanoPolyPhotonics, Geiselbergstr. 69, Potsdam-Golm 14476, Germany

Dominik Hühn

Philipp University of Marburg, Faculty of Physics, Biophotonics, Renthof 7, Marburg 35037, Germany

Wolfgang J. Parak

Philipp University of Marburg, Faculty of Physics, Biophotonics, Renthof 7, Marburg 35037, Germany

Niko Hildebrandt

University of Paris-South, Institute for Fundamental Electronics, NanoBioPhotonics, 15 Rue Georges Clémenceau, Orsay 91405, France

Hans-Gerd Löhmannsröben

University of Potsdam, Institute of Chemistry, Physical Chemistry, Karl-Liebknecht-Str. 24-25, Potsdam-Golm 14476, Germany

J. Biomed. Opt. 19(10), 101506 (Jul 02, 2014). doi:10.1117/1.JBO.19.10.101506
History: Received February 28, 2014; Revised June 3, 2014; Accepted June 4, 2014
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Abstract.  Nanobioconjugates have been synthesized using cadmium selenide quantum dots (QDs), europium complexes (EuCs), and biotin. In those conjugates, long-lived photoluminescence (PL) is provided by the europium complexes, which efficiently transfer energy via Förster resonance energy transfer (FRET) to the QDs in close spatial proximity. As a result, the conjugates have a PL emission spectrum characteristic for QDs combined with the long PL decay time characteristic for EuCs. The nanobioconjugates synthesis strategy and photophysical properties are described as well as their performance in a time-resolved streptavidin-biotin PL assay. In order to prepare the QD-EuC-biotin conjugates, first an amphiphilic polymer has been functionalized with the EuC and biotin. Then, the polymer has been brought onto the surface of the QDs (either QD655 or QD705) to provide functionality and to make the QDs water dispersible. Due to a short distance between EuC and QD, an efficient FRET can be observed. Additionally, the QD-EuC-biotin conjugates’ functionality has been demonstrated in a PL assay yielding good signal discrimination, both from autofluorescence and directly excited QDs. These newly designed QD-EuC-biotin conjugates expand the class of highly sensitive tools for bioanalytical optical detection methods for diagnostic and imaging applications.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Piotr J. Cywiński ; Tommy Hammann ; Dominik Hühn ; Wolfgang J. Parak ; Niko Hildebrandt, et al.
"Europium-quantum dot nanobioconjugates as luminescent probes for time-gated biosensing", J. Biomed. Opt. 19(10), 101506 (Jul 02, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.10.101506


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