Research Papers: Imaging

Delayed near-infrared analysis permits visualization of rodent retinal pigment epithelium layer in vivo

[+] Author Affiliations
Natalie Pankova

University of Toronto, Department of Laboratory Medicine and Pathobiology, 1 King’s College Circle, Toronto, Ontario M5S 1A8, Canada

University of Toronto, Department of Ophthalmology and Vision Sciences, 340 College Street, Toronto, Ontario M5T 3A9, Canada

St Michael’s Hospital, Keenan Research Centre for Biomedical Science, 209 Victoria Street, Toronto, Ontario M5B 1W8, Canada

Xu Zhao, Huiyuan Liang, Hai Wang

St Michael’s Hospital, Keenan Research Centre for Biomedical Science, 209 Victoria Street, Toronto, Ontario M5B 1W8, Canada

David Sung Hyeon Baek

University of Toronto, Department of Laboratory Medicine and Pathobiology, 1 King’s College Circle, Toronto, Ontario M5S 1A8, Canada

St Michael’s Hospital, Keenan Research Centre for Biomedical Science, 209 Victoria Street, Toronto, Ontario M5B 1W8, Canada

Shelley Boyd

University of Toronto, Department of Laboratory Medicine and Pathobiology, 1 King’s College Circle, Toronto, Ontario M5S 1A8, Canada

University of Toronto, Department of Ophthalmology and Vision Sciences, 340 College Street, Toronto, Ontario M5T 3A9, Canada

St Michael’s Hospital, Keenan Research Centre for Biomedical Science, 209 Victoria Street, Toronto, Ontario M5B 1W8, Canada

St. Michael’s Hospital, Department of Ophthalmology, 30 Bond Street, Toronto, Ontario M5B 1W8, Canada

McMaster University, Biomedical Engineering, 1280 Main Street West, Hamilton, Ontario L8S 4K1, Canada

J. Biomed. Opt. 19(7), 076007 (Jul 08, 2014). doi:10.1117/1.JBO.19.7.076007
History: Received March 25, 2014; Revised May 19, 2014; Accepted May 27, 2014
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Abstract.  Patches of atrophy of the retinal pigment epithelium (RPE) have not been described in rodent models of retinal degeneration, as they have the clinical setting using fundus autofluorescence. We hypothesize that prelabeling the RPE would increase contrast and allow for improved visualization of RPE loss in vivo. Here, we demonstrate a new technique termed “delayed near-infrared analysis (DNIRA)” that permits ready detection of rat RPE, using optical imaging in the near-infrared (IR) spectrum with aid of indocyanine green (ICG) dye. Using DNIRA, we demonstrate a fluorescent RPE signal that is detected using confocal scanning laser ophthalmoscopy up to 28 days following ICG injection. This signal is apparent only after ICG injection, is dose dependent, requires the presence of the ICG filters (795/810 nm excitation/emission), does not appear in the IR reflectance channel, and is eliminated in the presence of sodium iodate, a toxin that causes RPE loss. Rat RPE explants confirm internalization of ICG dye. Together with normal retinal electrophysiology, these findings demonstrate that DNIRA is a new and safe noninvasive optical imaging technique for in vivo visualization of the RPE in models of retinal disease.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Natalie Pankova ; Xu Zhao ; Huiyuan Liang ; David Sung Hyeon Baek ; Hai Wang, et al.
"Delayed near-infrared analysis permits visualization of rodent retinal pigment epithelium layer in vivo", J. Biomed. Opt. 19(7), 076007 (Jul 08, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.7.076007


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