Research Papers: Sensing

Accurate extraction of optical properties and top layer thickness of two-layered mucosal tissue phantoms from spatially resolved reflectance spectra

[+] Author Affiliations
Kung-Bin Sung

National Taiwan University, Graduate Institute of Biomedical Electronics and Bioinformatics, No. 1 Sec. 4 Roosevelt Road, Taipei 10617, Taiwan

National Taiwan University, Department of Electrical Engineering, No. 1 Sec. 4 Roosevelt Road, Taipei 10617, Taiwan

National Taiwan University, Molecular Imaging Center, No. 1 Sec. 4 Roosevelt Road, Taipei 10617, Taiwan

Kuang-Wei Shih

National Taiwan University, Graduate Institute of Biomedical Electronics and Bioinformatics, No. 1 Sec. 4 Roosevelt Road, Taipei 10617, Taiwan

Fang-Wei Hsu

National Taiwan University, Graduate Institute of Biomedical Electronics and Bioinformatics, No. 1 Sec. 4 Roosevelt Road, Taipei 10617, Taiwan

Hong-Po Hsieh

National Taiwan University, Graduate Institute of Biomedical Electronics and Bioinformatics, No. 1 Sec. 4 Roosevelt Road, Taipei 10617, Taiwan

Min-Jie Chuang

National Taiwan University, Graduate Institute of Biomedical Electronics and Bioinformatics, No. 1 Sec. 4 Roosevelt Road, Taipei 10617, Taiwan

Yi-Hsien Hsiao

National Taiwan University, Graduate Institute of Biomedical Electronics and Bioinformatics, No. 1 Sec. 4 Roosevelt Road, Taipei 10617, Taiwan

Yu-Hui Su

National Taiwan University, Graduate Institute of Biomedical Electronics and Bioinformatics, No. 1 Sec. 4 Roosevelt Road, Taipei 10617, Taiwan

Gen-Hao Tien

National Taiwan University, Graduate Institute of Biomedical Electronics and Bioinformatics, No. 1 Sec. 4 Roosevelt Road, Taipei 10617, Taiwan

J. Biomed. Opt. 19(7), 077002 (Jul 15, 2014). doi:10.1117/1.JBO.19.7.077002
History: Received March 20, 2014; Revised June 13, 2014; Accepted June 17, 2014
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Abstract.  We are reporting on an experimental investigation of a movable diffuse reflectance spectroscopy system to extract diagnostically relevant optical properties of two-layered tissue phantoms simulating mucosae that are covered with stratified squamous epithelium. The reflectance spectra were measured at multiple source-detector separations using two imaging fiber bundles in contact with the phantoms, one with its optical axis perpendicular to the sample surface (perpendicular probe) and the other with its distal end beveled and optical axis tilted at 45 deg (oblique probe). Polystyrene microspheres and purified human hemoglobin were used to make tissue phantoms whose scattering and absorption properties could be well controlled and theoretically predicted. Monte Carlo simulations were used to predict the reflectance spectra for system calibration and an iterative curve fitting that simultaneously extracted the top layer reduced scattering coefficient, thickness, bottom layer reduced scattering coefficient, and hemoglobin concentration of the phantoms. The errors of the recovered parameters ranged from 7% to 20%. The oblique probe showed higher accuracy in the extracted top layer reduced scattering coefficient and thickness than the perpendicular probe. The developed system and data analysis methods provide a feasible tool to quantify the optical properties in vivo.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Kung-Bin Sung ; Kuang-Wei Shih ; Fang-Wei Hsu ; Hong-Po Hsieh ; Min-Jie Chuang, et al.
"Accurate extraction of optical properties and top layer thickness of two-layered mucosal tissue phantoms from spatially resolved reflectance spectra", J. Biomed. Opt. 19(7), 077002 (Jul 15, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.7.077002


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