Research Papers: Imaging

Target-to-background enhancement in multispectral endoscopy with background autofluorescence mitigation for quantitative molecular imaging

[+] Author Affiliations
Chenying Yang

University of Washington, Department of Bioengineering, Seattle, Washington 98195, United States

Vivian W. Hou

University of Washington, Department of Biology, Seattle, Washington 98195, United States

Emily J. Girard

Fred Hutchinson Cancer Research Center, Clinical Research Division, Seattle, Washington 98109, United States

Leonard Y. Nelson, Eric J. Seibel

University of Washington, Department of Mechanical Engineering, Seattle, Washington 98195, United States

J. Biomed. Opt. 19(7), 076014 (Jul 15, 2014). doi:10.1117/1.JBO.19.7.076014
History: Received March 4, 2014; Revised June 18, 2014; Accepted June 25, 2014
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Abstract.  Fluorescence molecular imaging with exogenous probes improves specificity for the detection of diseased tissues by targeting unambiguous molecular signatures. Additionally, increased diagnostic sensitivity is expected with the application of multiple molecular probes. We developed a real-time multispectral fluorescence-reflectance scanning fiber endoscope (SFE) for wide-field molecular imaging of fluorescent dye-labeled molecular probes at nanomolar detection levels. Concurrent multichannel imaging with the wide-field SFE also allows for real-time mitigation of the background autofluorescence (AF) signal, especially when fluorescein, a U.S. Food and Drug Administration approved dye, is used as the target fluorophore. Quantitative tissue AF was measured for the ex vivo porcine esophagus and murine brain tissues across the visible and near-infrared spectra. AF signals were then transferred to the unit of targeted fluorophore concentration to evaluate the SFE detection sensitivity for sodium fluorescein and cyanine. Next, we demonstrated a real-time AF mitigation algorithm on a tissue phantom, which featured molecular probe targeted cells of high-grade dysplasia on a substrate containing AF species. The target-to-background ratio was enhanced by more than one order of magnitude when applying the real-time AF mitigation algorithm. Furthermore, a quantitative estimate of the fluorescein photodegradation (photobleaching) rate was evaluated and shown to be insignificant under the illumination conditions of SFE. In summary, the multichannel laser-based flexible SFE has demonstrated the capability to provide sufficient detection sensitivity, image contrast, and quantitative target intensity information for detecting small precancerous lesions in vivo.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Chenying Yang ; Vivian W. Hou ; Emily J. Girard ; Leonard Y. Nelson and Eric J. Seibel
"Target-to-background enhancement in multispectral endoscopy with background autofluorescence mitigation for quantitative molecular imaging", J. Biomed. Opt. 19(7), 076014 (Jul 15, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.7.076014


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