Research Papers: Sensing

In vivo measurement of tissue oxygenation by time-resolved luminescence spectroscopy: advantageous properties of dichlorotris(1, 10-phenanthroline)-ruthenium(II) hydrate

[+] Author Affiliations
Veronika Huntosova, Sandrine Gay, Senthil Kumar Rajendran, Matthieu Zellweger, Hubert van den Bergh, Georges Wagnières

Swiss Federal Institute of Technology (EPFL), Institute of Chemical Sciences and Engineering, Station 6, CH-1015 Lausanne, Switzerland

Patrycja Nowak-Sliwinska

Swiss Federal Institute of Technology (EPFL), Institute of Chemical Sciences and Engineering, Station 6, CH-1015 Lausanne, Switzerland

University Hospital (CHUV), Department of Urology, BH-10, Rue du Bugnon 46, CH-1011 Lausanne, Switzerland

J. Biomed. Opt. 19(7), 077004 (Jul 18, 2014). doi:10.1117/1.JBO.19.7.077004
History: Received March 25, 2014; Revised May 23, 2014; Accepted June 5, 2014
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Abstract.  Measuring tissue oxygenation in vivo is of interest in fundamental biological as well as medical applications. One minimally invasive approach to assess the oxygen partial pressure in tissue (pO2) is to measure the oxygen-dependent luminescence lifetime of molecular probes. The relation between tissue pO2 and the probes’ luminescence lifetime is governed by the Stern-Volmer equation. Unfortunately, virtually all oxygen-sensitive probes based on this principle induce some degree of phototoxicity. For that reason, we studied the oxygen sensitivity and phototoxicity of dichlorotris(1, 10-phenanthroline)-ruthenium(II) hydrate [Ru(Phen)] using a dedicated optical fiber–based, time-resolved spectrometer in the chicken embryo chorioallantoic membrane. We demonstrated that, after intravenous injection, Ru(Phen)’s luminescence lifetime presents an easily detectable pO2 dependence at a low drug dose (1mg/kg) and low fluence (120mJ/cm2 at 470 nm). The phototoxic threshold was found to be at 10J/cm2 with the same wavelength and drug dose, i.e., about two orders of magnitude larger than the fluence necessary to perform a pO2 measurement. Finally, an illustrative application of this pO2 measurement approach in a hypoxic tumor environment is presented.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Veronika Huntosova ; Sandrine Gay ; Patrycja Nowak-Sliwinska ; Senthil Kumar Rajendran ; Matthieu Zellweger, et al.
"In vivo measurement of tissue oxygenation by time-resolved luminescence spectroscopy: advantageous properties of dichlorotris(1, 10-phenanthroline)-ruthenium(II) hydrate", J. Biomed. Opt. 19(7), 077004 (Jul 18, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.7.077004


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