JBO Letters

Immobilization of gold nanoparticles on cell culture surfaces for safe and enhanced gold nanoparticle-mediated laser transfection

[+] Author Affiliations
Stefan Kalies, Dag Heinemann, Markus Schomaker, Lara Gentemann, Tammo Ripken

Laser Zentrum Hannover e.V., Department of Biomedical Optics, 30419 Hannover, Germany

Heiko Meyer

Laser Zentrum Hannover e.V., Department of Biomedical Optics, 30419 Hannover, Germany

Hannover Medical School, Department of Cardiothoracic, Transplantation and Vascular Surgery, 30625 Hannover, Germany

J. Biomed. Opt. 19(7), 070505 (Jul 28, 2014). doi:10.1117/1.JBO.19.7.070505
History: Received May 12, 2014; Revised June 20, 2014; Accepted July 2, 2014
Text Size: A A A

Abstract.  In comparison to standard transfection methods, gold nanoparticle-mediated laser transfection has proven to be a versatile alternative. This is based on its minor influence on cell viability and its high efficiency, especially for the delivery of small molecules like small interfering RNA. However, in order to transfer it to routine usage, a safety aspect is of major concern: The avoidance of nanoparticle uptake by the cells is desired. The immobilization of the gold nanoparticles on cell culture surfaces can address this issue. In this study, we achieved this by silanization of the appropriate surfaces and the binding of gold nanoparticles to them. Comparable perforation efficiencies to the previous approaches of gold nanoparticle-mediated laser transfection with free gold nanoparticles are demonstrated. The uptake of the immobilized particles by the cells is unlikely. Consequently, these investigations offer the possibility of bringing gold nanoparticle-mediated laser transfection closer to routine usage.

Figures in this Article
© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Stefan Kalies ; Dag Heinemann ; Markus Schomaker ; Lara Gentemann ; Heiko Meyer, et al.
"Immobilization of gold nanoparticles on cell culture surfaces for safe and enhanced gold nanoparticle-mediated laser transfection", J. Biomed. Opt. 19(7), 070505 (Jul 28, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.7.070505


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.