Research Papers: Imaging

Quantum dots targeted to vascular endothelial growth factor receptor 2 as a contrast agent for the detection of colorectal cancer

[+] Author Affiliations
Jordan L. Carbary-Ganz, Jennifer K. Barton, Urs Utzinger

University of Arizona, Biomedical Engineering GIDP, Tucson, Arizona 85721, United States

J. Biomed. Opt. 19(8), 086003 (Aug 06, 2014). doi:10.1117/1.JBO.19.8.086003
History: Received April 23, 2014; Revised July 17, 2014; Accepted July 21, 2014
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Abstract.  We successfully labeled colorectal cancer in vivo using quantum dots targeted to vascular endothelial growth factor receptor 2 (VEGFR2). Quantum dots with emission centered at 655 nm were bioconjugated to anti-VEGFR2 antibodies through streptavidin/biotin linking. The resulting QD655-VEGFR2 contrast agent was applied in vivo to the colon of azoxymethane (AOM) treated mice via lavage and allowed to incubate. The colons were then excised, cut longitudinally, opened to expose the lumen, and imaged en face using a fluorescence stereoscope. The QD655-VEGFR2 contrast agent produced a significant increase in contrast between diseased and undiseased tissues, allowing for fluorescence-based visualization of the diseased areas of the colon. Specificity was assessed by observing insignificant contrast increase when labeling colons of AOM-treated mice with quantum dots bioconjugated to isotype control antibodies, and by labeling the colons of saline-treated control mice. This contrast agent has a great potential for in vivo imaging of the colon through endoscopy.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Jordan L. Carbary-Ganz ; Jennifer K. Barton and Urs Utzinger
"Quantum dots targeted to vascular endothelial growth factor receptor 2 as a contrast agent for the detection of colorectal cancer", J. Biomed. Opt. 19(8), 086003 (Aug 06, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.8.086003


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