Research Papers: General

Additivity of light-scattering patterns of aggregated biological particles

[+] Author Affiliations
Alexander E. Moskalensky

Institute of Chemical Kinetics and Combustion SB RAS, Institutskaya 3, Novosibirsk 630090, Russia

Novosibirsk State University, Pirogova 2, Novosibirsk 630090, Russia

Dmitry I. Strokotov

Institute of Chemical Kinetics and Combustion SB RAS, Institutskaya 3, Novosibirsk 630090, Russia

Novosibirsk State University, Pirogova 2, Novosibirsk 630090, Russia

Andrei V. Chernyshev

Institute of Chemical Kinetics and Combustion SB RAS, Institutskaya 3, Novosibirsk 630090, Russia

Novosibirsk State University, Pirogova 2, Novosibirsk 630090, Russia

Valeri P. Maltsev

Institute of Chemical Kinetics and Combustion SB RAS, Institutskaya 3, Novosibirsk 630090, Russia

Novosibirsk State University, Pirogova 2, Novosibirsk 630090, Russia

Novosibirsk State Medical University, Krasny Prospect 52, Novosibirsk 630091, Russia

Maxim A. Yurkin

Institute of Chemical Kinetics and Combustion SB RAS, Institutskaya 3, Novosibirsk 630090, Russia

Novosibirsk State University, Pirogova 2, Novosibirsk 630090, Russia

J. Biomed. Opt. 19(8), 085004 (Aug 07, 2014). doi:10.1117/1.JBO.19.8.085004
History: Received May 11, 2014; Revised June 24, 2014; Accepted June 30, 2014
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Abstract.  The paper is focused on light scattering by aggregates of optically soft particles with a size larger than the wavelength, in particular, blood platelets. We conducted a systematic simulation of light scattering by dimers and larger aggregates of blood platelets, each modeled as oblate spheroids, using the discrete dipole approximation. Two-dimensional (2-D) light scattering patterns (LSPs) and internal fields showed that the multiple scattering between constituent particles can be neglected. Additionally, we derived conditions of the scattering angle and orientation of the dimer, under which the averaging of the 2-D LSPs over the azimuthal scattering angle washes out interference in the far field, resulting in averaged LSPs of the aggregate being equal to the sum of that for its constituents. We verified theoretical conclusions using the averaged LSPs of blood platelets measured with the scanning flow cytometer (SFC). Moreover, we obtained similar results for a model system of aggregates of polystyrene beads, studied both experimentally and theoretically. Finally, we discussed the potential of discriminating platelet aggregates from monomers using the SFC.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Alexander E. Moskalensky ; Dmitry I. Strokotov ; Andrei V. Chernyshev ; Valeri P. Maltsev and Maxim A. Yurkin
"Additivity of light-scattering patterns of aggregated biological particles", J. Biomed. Opt. 19(8), 085004 (Aug 07, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.8.085004


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