Research Papers: Imaging

Label-free real-time imaging of myelination in the Xenopus laevis tadpole by in vivo stimulated Raman scattering microscopy

[+] Author Affiliations
Chun-Rui Hu

University of Science and Technology of China, Hefei National Laboratory for Physical Sciences at Microscale, and the School of Life Sciences, Hefei 230027, China

Delong Zhang

Purdue University, Department of Chemistry, West Lafayette, Indiana 47907, United States

Mikhail N. Slipchenko

Purdue University, Weldon School of Biomedical Engineering, West Lafayette, Indiana 47907, United States

Ji-Xin Cheng

Purdue University, Department of Chemistry, West Lafayette, Indiana 47907, United States

Purdue University, Weldon School of Biomedical Engineering, West Lafayette, Indiana 47907, United States

Bing Hu

University of Science and Technology of China, Hefei National Laboratory for Physical Sciences at Microscale, and the School of Life Sciences, Hefei 230027, China

J. Biomed. Opt. 19(8), 086005 (Aug 07, 2014). doi:10.1117/1.JBO.19.8.086005
History: Received March 16, 2014; Revised July 9, 2014; Accepted July 10, 2014
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Abstract.  The myelin sheath plays an important role as the axon in the functioning of the neural system, and myelin degradation is a hallmark pathology of multiple sclerosis and spinal cord injury. Electron microscopy, fluorescent microscopy, and magnetic resonance imaging are three major techniques used for myelin visualization. However, microscopic observation of myelin in living organisms remains a challenge. Using a newly developed stimulated Raman scattering microscopy approach, we report noninvasive, label-free, real-time in vivo imaging of myelination by a single-Schwann cell, maturation of a single node of Ranvier, and myelin degradation in the transparent body of the Xenopus laevis tadpole.

Figures in this Article
© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Chun-Rui Hu ; Delong Zhang ; Mikhail N. Slipchenko ; Ji-Xin Cheng and Bing Hu
"Label-free real-time imaging of myelination in the Xenopus laevis tadpole by in vivo stimulated Raman scattering microscopy", J. Biomed. Opt. 19(8), 086005 (Aug 07, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.8.086005


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