Research Papers: General

Ultrasound-modulated fluorescence based on fluorescent microbubbles

[+] Author Affiliations
Yuan Liu

University of Texas at Arlington, Department of Bioengineering, Arlington, Texas 76010, United States

University of Texas at Arlington and University of Texas Southwestern Medical Center at Dallas, Joint Biomedical Engineering Program, Texas 75390, United States

Jameel A. Feshitan

University of Colorado, Department of Mechanical Engineering, Boulder, Colorado 80309-0427, United States

Ming-Yuan Wei

University of Texas at Arlington, Department of Bioengineering, Arlington, Texas 76010, United States

University of Texas at Arlington and University of Texas Southwestern Medical Center at Dallas, Joint Biomedical Engineering Program, Texas 75390, United States

Mark A. Borden

University of Colorado, Department of Mechanical Engineering, Boulder, Colorado 80309-0427, United States

Baohong Yuan

University of Texas at Arlington, Department of Bioengineering, Arlington, Texas 76010, United States

University of Texas at Arlington and University of Texas Southwestern Medical Center at Dallas, Joint Biomedical Engineering Program, Texas 75390, United States

J. Biomed. Opt. 19(8), 085005 (Aug 07, 2014). doi:10.1117/1.JBO.19.8.085005
History: Received April 15, 2014; Revised July 9, 2014; Accepted July 14, 2014
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Abstract.  Ultrasound-modulated fluorescence (UMF) imaging has been proposed to provide fluorescent contrast while maintaining ultrasound resolution in an optical-scattering medium (such as biological tissue). The major challenge is to extract the weakly modulated fluorescent signal from a bright and unmodulated background. UMF was experimentally demonstrated based on fluorophore-labeled microbubble contrast agents. These contrast agents were produced by conjugating N-hydroxysuccinimide (NHS)-ester-attached fluorophores on the surface of amine-functionalized microbubbles. The fluorophore surface concentration was controlled so that a significant self-quenching effect occurred when no ultrasound was applied. The intensity of the fluorescent emission was modulated when microbubbles were oscillated by ultrasound pulses, presented as UMF signal. Our results demonstrated that the UMF signals were highly dependent on the microbubbles’ oscillation amplitude and the initial surface fluorophore-quenching status. A maximum of 42% UMF modulation depth was achieved with a single microbubble under an ultrasound peak-to-peak pressure of 675 kPa. Further, UMF was detected from a 500-μm tube filled with contrast agents in water and scattering media with ultrasound resolution. These results indicate that ultrasound-modulated fluorescent microbubble contrast agents can potentially be used for fluorescence-based molecular imaging with ultrasound resolution in the future.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Yuan Liu ; Jameel A. Feshitan ; Ming-Yuan Wei ; Mark A. Borden and Baohong Yuan
"Ultrasound-modulated fluorescence based on fluorescent microbubbles", J. Biomed. Opt. 19(8), 085005 (Aug 07, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.8.085005


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