Research Papers: Imaging

Aberration correction during real time in vivo imaging of bone marrow with sensorless adaptive optics confocal microscope

[+] Author Affiliations
Zhibin Wang

Chinese Academy of Sciences, The Key Laboratory on Adaptive Optics, Chengdu 610209, China

Chinese Academy of Sciences, Institute of Optics and Electronics, The Laboratory on Adaptive Optics, Chengdu 610209, China

University of Chinese Academy of Sciences, Beijing 100039, China

Dan Wei, Xunbin Wei

Shanghai Jiao Tong University, School of Biomedical Engineering and Med-X Research Institute, 1954 Huashan Road, Shanghai 200030, China

Ling Wei, Yi He, Guohua Shi, Yudong Zhang

Chinese Academy of Sciences, The Key Laboratory on Adaptive Optics, Chengdu 610209, China

Chinese Academy of Sciences, Institute of Optics and Electronics, The Laboratory on Adaptive Optics, Chengdu 610209, China

J. Biomed. Opt. 19(8), 086009 (Aug 12, 2014). doi:10.1117/1.JBO.19.8.086009
History: Received April 4, 2014; Revised July 10, 2014; Accepted July 11, 2014
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Abstract.  We have demonstrated adaptive correction of specimen-induced aberration during in vivo imaging of mouse bone marrow vasculature with confocal fluorescence microscopy. Adaptive optics system was completed with wavefront sensorless correction scheme based on stochastic parallel gradient descent algorithm. Using image sharpness as the optimization metric, aberration correction was performed based upon Zernike polynomial modes. The experimental results revealed the improved signal and resolution leading to a substantially enhanced image contrast with aberration correction. The image quality of vessels at 38- and 75-μm depth increased three times and two times, respectively. The corrections allowed us to detect clearer bone marrow vasculature structures at greater contrast and improve the signal-to-noise ratio.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Zhibin Wang ; Dan Wei ; Ling Wei ; Yi He ; Guohua Shi, et al.
"Aberration correction during real time in vivo imaging of bone marrow with sensorless adaptive optics confocal microscope", J. Biomed. Opt. 19(8), 086009 (Aug 12, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.8.086009


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