Research Papers: General

Phantom validation of Monte Carlo modeling for noncontact depth sensitive fluorescence measurements in an epithelial tissue model

[+] Author Affiliations
Yi Hong Ong

Nanyang Technological University, School of Chemical and Biomedical Engineering, Division of Bioengineering, Singapore 637457, Singapore

Caigang Zhu

Nanyang Technological University, School of Chemical and Biomedical Engineering, Division of Bioengineering, Singapore 637457, Singapore

Quan Liu

Nanyang Technological University, School of Chemical and Biomedical Engineering, Division of Bioengineering, Singapore 637457, Singapore

J. Biomed. Opt. 19(8), 085006 (Aug 12, 2014). doi:10.1117/1.JBO.19.8.085006
History: Received May 22, 2014; Revised July 18, 2014; Accepted July 21, 2014
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Abstract.  Experimental investigation and optimization of various optical parameters in the design of depth sensitive optical measurements in layered tissues would require a huge amount of time and resources. A computational method to model light transport in layered tissues using Monte Carlo simulations has been developed for decades to reduce the cost incurred during this process. In this work, we employed the Monte Carlo method to investigate the depth sensitivity achieved by various illumination and detection configurations including both the traditional cone configurations and new cone shell configurations, which are implemented by convex or axicon lenses. Phantom experiments have been carried out to validate the Monte Carlo modeling of fluorescence in a two-layered turbid, epithelial tissue model. The measured fluorescence and depth sensitivity of different illumination–detection configurations were compared with each other. The results indicate excellent agreement between the experimental and simulation results in the trends of fluorescence intensity and depth sensitivity. The findings of this study and the development of the Monte Carlo method for noncontact setups provide useful insight and assistance in the planning and optimization of optical designs for depth sensitive fluorescence measurements.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Yi Hong Ong ; Caigang Zhu and Quan Liu
"Phantom validation of Monte Carlo modeling for noncontact depth sensitive fluorescence measurements in an epithelial tissue model", J. Biomed. Opt. 19(8), 085006 (Aug 12, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.8.085006


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