Research Papers: Sensing

Polarized Raman spectroscopic investigations on hemoglobin ordering in red blood cells

[+] Author Affiliations
Sunita Ahlawat, Aniket Chowdhury, Nitin Kumar, Abha Uppal, Ravi Shanker Verma, Pradeep Kumar Gupta

Raja Ramanna Centre for Advanced Technology, Laser Biomedical Applications and Instrumentation Division, Indore, 452013, India

J. Biomed. Opt. 19(8), 087002 (Aug 14, 2014). doi:10.1117/1.JBO.19.8.087002
History: Received May 8, 2014; Revised July 7, 2014; Accepted July 17, 2014
Text Size: A A A

Abstract.  We have investigated the dependence of the Raman spectrum of an optically trapped red blood cell (RBC) on the orientation of the cell, relative to the polarization direction of the Raman excitation beam. The Raman scattered light polarized parallel to the polarization direction of the excitation beam was observed to depend upon the orientation of the cell. In particular, the heme bands at 754cm1 and in the 1500 to 1700cm1 region were observed to become maximum when the cells’ equatorial plane was parallel to the excitation beam polarization direction and minimum when the cells’ plane was normal to the polarization direction. In contrast, no significant orientational dependence was seen in the Raman scattered light polarized orthogonal to the polarization direction of the excitation beam. Theoretical simulations carried out to investigate these observations suggest that inside the RBCs, the hemoglobin molecules must be present in an ordered arrangement, such that heme-porphyrin planes become preferentially orientated parallel to the RBCs’ equatorial plane.

Figures in this Article
© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Sunita Ahlawat ; Aniket Chowdhury ; Nitin Kumar ; Abha Uppal ; Ravi Shanker Verma, et al.
"Polarized Raman spectroscopic investigations on hemoglobin ordering in red blood cells", J. Biomed. Opt. 19(8), 087002 (Aug 14, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.8.087002


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.