Research Papers: Sensing

Waveband selection of reagent-free determination for thalassemia screening indicators using Fourier transform infrared spectroscopy with attenuated total reflection

[+] Author Affiliations
Xiaoli Long

Jinan University, Key Laboratory of Optoelectronic Information and Sensing Technologies of Guangdong Higher Educational Institutes, Huangpu Road West 601, Tianhe District, Guangzhou 510632, China

Guisong Liu

Jinan University, Key Laboratory of Optoelectronic Information and Sensing Technologies of Guangdong Higher Educational Institutes, Huangpu Road West 601, Tianhe District, Guangzhou 510632, China

Tao Pan

Jinan University, Key Laboratory of Optoelectronic Information and Sensing Technologies of Guangdong Higher Educational Institutes, Huangpu Road West 601, Tianhe District, Guangzhou 510632, China

Jiemei Chen

Jinan University, Key Laboratory of Optoelectronic Information and Sensing Technologies of Guangdong Higher Educational Institutes, Huangpu Road West 601, Tianhe District, Guangzhou 510632, China

J. Biomed. Opt. 19(8), 087004 (Aug 19, 2014). doi:10.1117/1.JBO.19.8.087004
History: Received June 2, 2014; Revised July 24, 2014; Accepted July 25, 2014
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Abstract.  A reagent-free determination method for the thalassemia screening indicators hemoglobin (Hb), mean corpuscular Hb (MCH), and mean corpuscular volume (MCV) was developed based on Fourier transform infrared spectrometers equipped with an attenuated total reflection accessory. A random and stability-dependent rigorous process of calibration, prediction, and validation was conducted. Appropriate wavebands were selected using the improved moving window partial least squares method with stability and equivalence. The obtained optimal wavebands were 1722 to 1504cm1 for Hb, 1653 to 901cm1 for MCH, and 1562 to 964cm1 for MCV. A model set equivalent to the optimal model was proposed for each indicator; the public waveband of Hb equivalent wavebands was 1717 to 1510cm1, and the public equivalent waveband for MCH and MCV was 1562 to 901cm1. All selected wavebands were within the MIR fingerprint region and achieved high validation effects. The sensitivity and specificity were 100.0% and 96.9% for the optimal wavebands and 100.0% and 95.3% for the equivalent wavebands, respectively. Thus, the spectral prediction was highly accurate for determining negative and positive for thalassemia screening. This technique is rapid and simple in comparison with conventional methods and is a promising tool for thalassemia screening in large populations.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Xiaoli Long ; Guisong Liu ; Tao Pan and Jiemei Chen
"Waveband selection of reagent-free determination for thalassemia screening indicators using Fourier transform infrared spectroscopy with attenuated total reflection", J. Biomed. Opt. 19(8), 087004 (Aug 19, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.8.087004


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