JBO Letters

Time-resolved fluorescence spectroscopy and ultrasound backscatter microscopy for nondestructive evaluation of vascular grafts

[+] Author Affiliations
Hussain Fatakdawala, Laura Marcu

University of California Davis, Department of Biomedical Engineering, 451 East Health Sciences Drive, Davis, California 95616, United States

Leigh G. Griffiths

University of California Davis, Department of Medicine and Epidemiology, VM2 Building, Davis, California 95618, United States

Sterling Humphrey

University of California Davis, Department of Surgery, 2221 Stockton Boulevard, Sacramento, California 95817, United States

J. Biomed. Opt. 19(8), 080503 (Aug 22, 2014). doi:10.1117/1.JBO.19.8.080503
History: Received May 31, 2014; Revised July 28, 2014; Accepted August 1, 2014
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Abstract.  Quantitative and qualitative evaluations of structure and composition are important in monitoring development of engineered vascular tissue both in vitro and in vivo. Destructive techniques are an obstacle for performing time-lapse analyses from a single sample or animal. This study demonstrates the ability of time-resolved fluorescence spectroscopy (TRFS) and ultrasound backscatter microscopy (UBM), as nondestructive and synergistic techniques, for compositional and morphological analyses of tissue grafts, respectively. UBM images and integrated backscatter coefficients demonstrate the ability to visualize and quantify postimplantation changes in vascular graft biomaterials such as loss of the external elastic lamina and intimal/medial thickening over the grafted region as well as graft integration with the surrounding tissue. TRFS results show significant changes in spectra, average lifetime, and fluorescence decay parameters owing to changes in collagen, elastin, and cellular content between normal and grafted tissue regions. These results lay the foundation for the application of a catheter-based technique for in vivo evaluation of vascular grafts using TRFS and UBM.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Hussain Fatakdawala ; Leigh G. Griffiths ; Sterling Humphrey and Laura Marcu
"Time-resolved fluorescence spectroscopy and ultrasound backscatter microscopy for nondestructive evaluation of vascular grafts", J. Biomed. Opt. 19(8), 080503 (Aug 22, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.8.080503


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