Research Papers: Imaging

Development of human serum albumin conjugated with near-infrared dye for photoacoustic tumor imaging

[+] Author Affiliations
Kengo Kanazaki, Atsushi Takahashi

Canon Inc., Medical Imaging Project, Corporate R&D Headquarters, 3-30-2 Shimomaruko, Ohta-ku, Tokyo 146-8501, Japan

Kohei Sano

Kyoto University, Department of Patho-Functional Bioanalysis Graduate School of Pharmaceutical Sciences, 46-29 Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan

Kyoto University Hospital, 54 Kawaharacho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan

Akira Makino

Kyoto University, Department of Patho-Functional Bioanalysis Graduate School of Pharmaceutical Sciences, 46-29 Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan

University of Fukui, Biomedical Imaging Research Center, 23-3 Matsuokashimoaizuki, Eiheiji-cho, Yoshida-gun, Fukui 910-1193, Japan

Jun Deguchi, Manami Ohashi, Takashi Temma, Masahiro Ono, Hideo Saji

Kyoto University, Department of Patho-Functional Bioanalysis Graduate School of Pharmaceutical Sciences, 46-29 Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan

J. Biomed. Opt. 19(9), 096002 (Sep 05, 2014). doi:10.1117/1.JBO.19.9.096002
History: Received March 31, 2014; Revised August 9, 2014; Accepted August 12, 2014
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Abstract.  Photoacoustic (PA) imaging has emerged as a noninvasive diagnostic method which detects ultrasonic waves thermoelastically induced by optical absorbers irradiated with laser. For tumor diagnosis, PA contrast agent has been proposed to enhance the PA effect for detecting tumors sensitively. Here, we prepared a human serum albumin (HSA) conjugated with indocyanine green (ICG) as a PA contrast agent allowing enhanced permeability and retention effect for sensitive tumor imaging. The feasibility of PA imaging with HSA-ICG to detect allografted tumors was evaluated in tumor-bearing mice. In vivo fluorescence imaging and radiolabeled biodistribution study showed that the biodistribution dramatically changed as the number of ICG bound to HSA increased, and the maximum accumulation of ICG was achieved when around three ICG molecules were loaded on an HSA. In vivo PA imaging demonstrated a tumor-selective and dose-dependent increase of PA signal intensity in mice injected with HSA-ICG (R2=0.88, 387% increase for HSA-ICG, 104 nmol ICG). In conclusion, HSA-ICG clearly visualized the allografted tumors with high tumor-to-background ratios having high quantitative and spatial resolution for the sensitive PA imaging of tumors. HSA-ICG could be useful as a favorable contrast agent for PA tumor imaging for the management of cancer.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Kengo Kanazaki ; Kohei Sano ; Akira Makino ; Atsushi Takahashi ; Jun Deguchi, et al.
"Development of human serum albumin conjugated with near-infrared dye for photoacoustic tumor imaging", J. Biomed. Opt. 19(9), 096002 (Sep 05, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.9.096002


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