Our data show that the calculation of the two AGEs indicators described above was leveled to constant values for the contact zones of the three age-groups. In addition, in the case of the old adult contact zone, a twofold to threefold drastic reduction of these indicators was observed compared with the intact collagen age-groups, which tends to suggest a masking of the biochemical spectral information. At the level of the protein structure, from the difference spectra, it appears that the old adult and newborn collagen contact zones exhibit a quite similar profile in the corresponding bands, whereas for the young adult, a perturbation of its secondary structure is noticed as witnessed by the changes observed at the level of the Amide I, II, and III bands. These findings are interesting and to the best of our knowledge have not been previously reported. The mechanisms of interaction at the level of these contact zones and as a function of age appear quite complex and require other complementary investigations for understanding them. Methods like Raman microspectroscopy could produce complementary vibrational information and other biophotonic approaches like polarized second harmonic generation could help to image the cell/collagen contact zones and inform on morphological parameters, such as the molecular order, the degree of orientational freedom, the molecular constraint, and rigidity of the collagen fibers.38 Indeed, these should be supported by conventional biochemical analysis.