Research Papers: Imaging

Spatial mapping of drug delivery to brain tissue using hyperspectral spatial frequency-domain imaging

[+] Author Affiliations
Rajinder P. Singh-Moon

Columbia University College of Physicians and Surgeons, Department of Anesthesiology, 630 West 168th Street, New York, New York 10032, United States

Darren M. Roblyer

Boston University, Department of Biomedical Engineering, 44 Cummington Street, Boston, Massachusetts 02215, United States

Irving J. Bigio

Boston University, Department of Biomedical Engineering, 44 Cummington Street, Boston, Massachusetts 02215, United States

Boston University, Department of Electrical Engineering, 44 Cummington Street, Boston, Massachusetts 02215, United States

Shailendra Joshi

Columbia University College of Physicians and Surgeons, Department of Anesthesiology, 630 West 168th Street, New York, New York 10032, United States

J. Biomed. Opt. 19(9), 096003 (Sep 08, 2014). doi:10.1117/1.JBO.19.9.096003
History: Received May 13, 2014; Revised August 6, 2014; Accepted August 15, 2014
Text Size: A A A

Abstract.  We present an application of spatial frequency-domain imaging (SFDI) to the wide-field imaging of drug delivery to brain tissue. Measurements were compared with values obtained by a previously validated variation of diffuse reflectance spectroscopy, the method of optical pharmacokinetics (OP). We demonstrate a cross-correlation between the two methods for absorption extraction and drug concentration determination in both experimental tissue phantoms and freshly extracted rodent brain tissue. These methods were first used to assess intra-arterial (IA) delivery of cationic liposomes to brain tissue in Sprague Dawley rats under transient cerebral hypoperfusion. Results were found to be in agreement with previously published experimental data and pharmacokinetic models of IA drug delivery. We then applied the same scheme to evaluate IA mitoxantrone delivery to glioma-bearing rats. Good correlation was seen between OP and SFDI determined concentrations taken from normal and tumor averaged sites. This study shows the feasibility of mapping drug/tracer distributions and encourages the use of SFDI for spatial imaging of tissues for drug/tracer-tagged carrier deposition and pharmacokinetic studies.

Figures in this Article
© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Rajinder P. Singh-Moon ; Darren M. Roblyer ; Irving J. Bigio and Shailendra Joshi
"Spatial mapping of drug delivery to brain tissue using hyperspectral spatial frequency-domain imaging", J. Biomed. Opt. 19(9), 096003 (Sep 08, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.9.096003


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.