JBO Letters

Preclinical evaluation of a novel cyanine dye for tumor imaging with in vivo photoacoustic imaging

[+] Author Affiliations
Takashi Temma, Satoru Onoe, Masahiro Ono, Hideo Saji

Kyoto University, Graduate School of Pharmaceutical Sciences, Department of Patho-Functional Bioanalysis, 46-29 Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan

Kengo Kanazaki

Kyoto University, Graduate School of Pharmaceutical Sciences, Department of Patho-Functional Bioanalysis, 46-29 Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan

Canon Inc., Medical Imaging Project, Corporate R&D Headquarters, 3-30-2 Shimomaruko, Ohta-ku, Tokyo 146-8501, Japan

J. Biomed. Opt. 19(9), 090501 (Sep 08, 2014). doi:10.1117/1.JBO.19.9.090501
History: Received July 2, 2014; Revised August 11, 2014; Accepted August 18, 2014
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Abstract.  Photoacoustic imaging (PA imaging or PAI) has shown great promise in the detection and monitoring of cancer. Although nanocarrier-based contrast agents have been studied for use in PAI, small molecule contrast agents are required due to their ease of preparation, cost-effectiveness, and low toxicity. Here, we evaluated the usefulness of a novel cyanine dye IC7-1-Bu as a PAI contrast agent without conjugated targeting moieties for in vivo tumor imaging in a mice model. Basic PA characteristics of IC7-1-Bu were compared with indocyanine green (ICG), a Food and Drug Administration approved dye, in an aqueous solution. We evaluated the tumor accumulation profile of IC7-1-Bu and ICG by in vivo fluorescence imaging. In vivo PAI was then performed with a photoacoustic tomography system 24 and 48 h after intravenous injection of IC7-1-Bu into tumor bearing mice. IC7-1-Bu showed about a 2.3-fold higher PA signal in aqueous solution compared with that of ICG. Unlike ICG, IC7-1-Bu showed high tumor fluorescence after intravenous injection. In vivo PAI provided a tumor to background PA signal ratio of approximately 2.5 after intravenous injection of IC7-1-Bu. These results indicate that IC7-1-Bu is a promising PAI contrast agent for cancer imaging without conjugation of targeting moieties.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Takashi Temma ; Satoru Onoe ; Kengo Kanazaki ; Masahiro Ono and Hideo Saji
"Preclinical evaluation of a novel cyanine dye for tumor imaging with in vivo photoacoustic imaging", J. Biomed. Opt. 19(9), 090501 (Sep 08, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.9.090501


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