Research Papers: Therapeutic

Low-level laser therapy on skeletal muscle inflammation: evaluation of irradiation parameters

[+] Author Affiliations
Matías Mantineo

University of Coimbra, Instrumentation Center, Department of Physics, Coimbra 3004-516, Portugal

IBILI-Institute for Biomedical Imaging and Life Sciences, Azinhaga de Santa Comba-Celas, Coimbra 3000-548, Portugal

João P. Pinheiro

University of Coimbra, Faculty of Medicine, Azinhaga de Santa Comba-Celas, Coimbra 3000-548, Portugal

António M. Morgado

University of Coimbra, Instrumentation Center, Department of Physics, Coimbra 3004-516, Portugal

IBILI-Institute for Biomedical Imaging and Life Sciences, Azinhaga de Santa Comba-Celas, Coimbra 3000-548, Portugal

J. Biomed. Opt. 19(9), 098002 (Sep 08, 2014). doi:10.1117/1.JBO.19.9.098002
History: Received March 31, 2014; Revised August 5, 2014; Accepted August 19, 2014
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Abstract.  We evaluated the effect of different irradiation parameters in low-level laser therapy (LLLT) for treating inflammation induced in the gastrocnemius muscle of rats through cytokines concentration in systemic blood and analysis of muscle tissue. We used continuous (830 and 980 nm) and pulsed illuminations (830 nm). Animals were divided into five groups per wavelength (10, 20, 30, 40, and 50 mW), and a control group. LLLT was applied during 5 days with a constant irradiation time and area. TNF-α, IL-1β, IL-2, and IL-6 cytokines were quantified by ELISA. Inflammatory cells were counted using microscopy. Identical methodology was used with pulsed illumination. Average power (40 mW) and duty cycle were kept constant (80%) at five frequencies (5, 25, 50, 100, and 200 Hz). For continuous irradiation, treatment effects occurred for all doses, with a reduction of TNF-α, IL-1β, and IL-6 cytokines and inflammatory cells. Continuous irradiation at 830 nm was more effective, a result explained by the action spectrum of cytochrome c oxidase (CCO). Best results were obtained for 40 mW, with data suggesting a biphasic dose response. Pulsed wave irradiation was only effective for higher frequencies, a result that might be related to the rate constants of the CCO internal electron transfer process.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Matías Mantineo ; João P. Pinheiro and António M. Morgado
"Low-level laser therapy on skeletal muscle inflammation: evaluation of irradiation parameters", J. Biomed. Opt. 19(9), 098002 (Sep 08, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.9.098002


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