Research Papers: Sensing

Thermal evaluation of laser exposures in an in vitro retinal model by microthermal sensing

[+] Author Affiliations
Tae Y. Choi, Ramesh Shrestha

University of North Texas, Department of Mechanical and Energy Engineering, 3940 N. Elm Street Denton, Texas 76207, United States

Michael L. Denton, Gary D. Noojin

TASC, Inc., Department of Biomedical Sciences and Technologies, 4141 Petroleum Road, JBSA Fort Sam Houston, Texas 78234, United States

Larry E. Estlack

Conceptual MindWorks, Inc., 4141 Petroleum Road, JBSA Fort Sam Houston, Texas 78234, United States

Benjamin A. Rockwell, Robert Thomas

711th Human Performance Wing, Bioeffects Division, Optical Radiation Bioeffects Branch, 4141 Petroleum Road, JBSA Fort Sam Houston, Texas 78234, United States

Dongsik Kim

POSTECH, Department of Mechanical Engineering, San 31 Hyoja-Dong, Nam-Gu, Pohang, Gyungbuk 790-784, Republic of Korea

J. Biomed. Opt. 19(9), 097003 (Sep 15, 2014). doi:10.1117/1.JBO.19.9.097003
History: Received April 10, 2014; Revised July 18, 2014; Accepted August 25, 2014
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Abstract.  A temperature detection system using a micropipette thermocouple sensor was developed for use within mammalian cells during laser exposure with an 8.6-μm beam at 532 nm. We have demonstrated the capability of measuring temperatures at a single-cell level in the microscale range by inserting micropipette-based thermal sensors of size ranging from 2 to 4μm into the membrane of a live retinal pigment epithelium (RPE) cell subjected to a laser beam. We setup the treatment groups of 532-nm laser-irradiated single RPE cell and in situ temperature recordings were made over time. Thermal profiles are given for representative cells experiencing damage resulting from exposures of 0.2 to 2 s. The measured maximum temperature rise for each cell ranges from 39 to 73°C; the RPE cells showed a signature of death for all the cases reported herein. In order to check the cell viability, real-time fluorescence microscopy was used to identify the transition of pigmented RPE cells between viable and damaged states due to laser exposure.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Tae Y. Choi ; Michael L. Denton ; Gary D. Noojin ; Larry E. Estlack ; Ramesh Shrestha, et al.
"Thermal evaluation of laser exposures in an in vitro retinal model by microthermal sensing", J. Biomed. Opt. 19(9), 097003 (Sep 15, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.9.097003


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