Research Papers: Imaging

Induction and imaging of photothrombotic stroke in conscious and freely moving rats

[+] Author Affiliations
Hongyang Lu

Shanghai Jiao Tong University, School of Biomedical Engineering, 800 Dongchuan Road, Shanghai 200240, China

Shanghai Jiao Tong University, Med-X Research Institute, 1954 Huashan Road, Shanghai 200030, China

Yao Li

Shanghai Jiao Tong University, School of Biomedical Engineering, 800 Dongchuan Road, Shanghai 200240, China

Shanghai Jiao Tong University, Med-X Research Institute, 1954 Huashan Road, Shanghai 200030, China

Lu Yuan

Shanghai Jiao Tong University, Med-X Research Institute, 1954 Huashan Road, Shanghai 200030, China

Hangdao Li

Shanghai Jiao Tong University, Med-X Research Institute, 1954 Huashan Road, Shanghai 200030, China

Xiaodan Lu

Shanghai Jiao Tong University, Med-X Research Institute, 1954 Huashan Road, Shanghai 200030, China

Shanbao Tong

Shanghai Jiao Tong University, School of Biomedical Engineering, 800 Dongchuan Road, Shanghai 200240, China

Shanghai Jiao Tong University, Med-X Research Institute, 1954 Huashan Road, Shanghai 200030, China

J. Biomed. Opt. 19(9), 096013 (Sep 19, 2014). doi:10.1117/1.JBO.19.9.096013
History: Received June 15, 2014; Revised August 26, 2014; Accepted August 28, 2014
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Abstract.  In experimental stroke research, anesthesia is common and serves as a major reason for translational failure. Real-time cerebral blood flow (CBF) monitoring during stroke onset can provide important information for the prediction of brain injury; however, this is difficult to achieve in clinical practice due to various technical problems. We created a photothrombotic focal ischemic stroke model utilizing our self-developed miniature headstage in conscious and freely moving rats. In this model, a high spatiotemporal resolution imager using laser speckle contrast imaging technology was integrated to acquire real-time two-dimensional CBF information during thrombosis. The feasibility, stability, and reliability of the system were tested in terms of CBF, behavior, and T2-weighted magnetic resonance imaging (MRI) findings. After completion of occlusion, the CBF in the targeted cortex of the stroke group was reduced to 16±9% of the baseline value. The mean infarct volume measured by MRI 24 h postmodeling was 77±11mm3 and correlated well with CBF (R2=0.74). This rodent model of focal cerebral ischemia and real-time blood flow imaging opens the possibility of performing various fundamental and translational studies on stroke without the influence of anesthetics.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Hongyang Lu ; Yao Li ; Lu Yuan ; Hangdao Li ; Xiaodan Lu, et al.
"Induction and imaging of photothrombotic stroke in conscious and freely moving rats", J. Biomed. Opt. 19(9), 096013 (Sep 19, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.9.096013


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