Research Papers: Sensing

Photonic detection and characterization of DNA using sapphire microspheres

[+] Author Affiliations
Mohammed Sharif Murib

Hasselt University, Instituut voor Materiaalonderzoek, Wetenschapspark 1, B-3590 Diepenbeek, Belgium

Weng-Siang Yeap

Hasselt University, Instituut voor Materiaalonderzoek, Wetenschapspark 1, B-3590 Diepenbeek, Belgium

Daan Martens

Ghent University—Information Technology, Department of Information Technology, Photonics Research Group, Sint-Pietersnieuwstraat 41, B-9000 Gent, Belgium

Peter Bienstman

Ghent University—Information Technology, Department of Information Technology, Photonics Research Group, Sint-Pietersnieuwstraat 41, B-9000 Gent, Belgium

Ward De Ceuninck

Hasselt University, Instituut voor Materiaalonderzoek, Wetenschapspark 1, B-3590 Diepenbeek, Belgium

Interuniversitair Microelectronica Centrum vereniging zonder winstoogmerk, Division Instituut voor Materiaalonderzoek in de Micro-Elektronica, Wetenschapspark 1, B-3590 Diepenbeek, Belgium

Bart van Grinsven

Hasselt University, Instituut voor Materiaalonderzoek, Wetenschapspark 1, B-3590 Diepenbeek, Belgium

Interuniversitair Microelectronica Centrum vereniging zonder winstoogmerk, Division Instituut voor Materiaalonderzoek in de Micro-Elektronica, Wetenschapspark 1, B-3590 Diepenbeek, Belgium

Michael J. Schöning

Aachen University of Applied Sciences, Institute of Nano- and Biotechnologies, Heinrich-Mußmann-Straße 1, D-52428 Jülich, Germany

Luc Michiels

Hasselt University, BIOMED, Agoralaan Building C, B-3590 Diepenbeek, Belgium

Ken Haenen

Hasselt University, Instituut voor Materiaalonderzoek, Wetenschapspark 1, B-3590 Diepenbeek, Belgium

Interuniversitair Microelectronica Centrum vereniging zonder winstoogmerk, Division Instituut voor Materiaalonderzoek in de Micro-Elektronica, Wetenschapspark 1, B-3590 Diepenbeek, Belgium

Marcel Ameloot

Hasselt University, BIOMED, Agoralaan Building C, B-3590 Diepenbeek, Belgium

Ali Serpengüzel

Koç University, Department of Physics, Microphotonics Research Laboratory, Rumelifeneri Yolu, Sarıyer, Istanbul 34450, Turkey

Patrick Wagner

Hasselt University, Instituut voor Materiaalonderzoek, Wetenschapspark 1, B-3590 Diepenbeek, Belgium

Interuniversitair Microelectronica Centrum vereniging zonder winstoogmerk, Division Instituut voor Materiaalonderzoek in de Micro-Elektronica, Wetenschapspark 1, B-3590 Diepenbeek, Belgium

J. Biomed. Opt. 19(9), 097006 (Sep 26, 2014). doi:10.1117/1.JBO.19.9.097006
History: Received May 21, 2014; Revised September 2, 2014; Accepted September 3, 2014
Text Size: A A A

Abstract.  A microcavity-based deoxyribonucleic acid (DNA) optical biosensor is demonstrated for the first time using synthetic sapphire for the optical cavity. Transmitted and elastic scattering intensity at 1510 nm are analyzed from a sapphire microsphere (radius 500μm, refractive index 1.77) on an optical fiber half coupler. The 0.43 nm angular mode spacing of the resonances correlates well with the optical size of the sapphire sphere. Probe DNA consisting of a 36-mer fragment was covalently immobilized on a sapphire microsphere and hybridized with a 29-mer target DNA. Whispering gallery modes (WGMs) were monitored before the sapphire was functionalized with DNA and after it was functionalized with single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). The shift in WGMs from the surface modification with DNA was measured and correlated well with the estimated thickness of the add-on DNA layer. It is shown that ssDNA is more uniformly oriented on the sapphire surface than dsDNA. In addition, it is shown that functionalization of the sapphire spherical surface with DNA does not affect the quality factor (Q104) of the sapphire microspheres. The use of sapphire is especially interesting because this material is chemically resilient, biocompatible, and widely used for medical implants.

Figures in this Article
© 2014 Society of Photo-Optical Instrumentation Engineers

Topics

Sapphire ; Scattering

Citation

Mohammed Sharif Murib ; Weng-Siang Yeap ; Daan Martens ; Peter Bienstman ; Ward De Ceuninck, et al.
"Photonic detection and characterization of DNA using sapphire microspheres", J. Biomed. Opt. 19(9), 097006 (Sep 26, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.9.097006


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