Research Papers: Imaging

Vertically scanned laser sheet microscopy

[+] Author Affiliations
Di Dong

Chinese Academy of Sciences, Key Laboratory of Molecular Imaging, Beijing 100190, China

Alicia Arranz

Swiss Federal Institute of Technology (ETH-Zurich), Institute for Biomedical Engineering, Switzerland

Shouping Zhu

Xidian University, Xian, School of Life Science and Technology, Shaanxi 710071, China

Yujie Yang

Chinese Academy of Sciences, Key Laboratory of Molecular Imaging, Beijing 100190, China

Liangliang Shi

Chinese Academy of Sciences, Key Laboratory of Molecular Imaging, Beijing 100190, China

Jun Wang

Harbin University of Science and Technology, School of Automation, Harbin 150080, China

Chen Shen

Xidian University, Xian, School of Life Science and Technology, Shaanxi 710071, China

Jie Tian

Chinese Academy of Sciences, Key Laboratory of Molecular Imaging, Beijing 100190, China

Jorge Ripoll

Universidad Carlos III of Madrid, Department of Bioengineering and Aerospace Engineering, Madrid 28911, Spain

Instituto de Investigación Sanitaria del Hospital Gregorio Marañón, Experimental Medicine and Surgery Unit, Madrid 28007, Spain

Foundation for Research and Technology-Hellas, Institute of Electronic Structure and Laser, Heraklion 71110, Greece

J. Biomed. Opt. 19(10), 106001 (Oct 01, 2014). doi:10.1117/1.JBO.19.10.106001
History: Received June 22, 2014; Revised August 14, 2014; Accepted September 2, 2014
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Abstract.  Laser sheet microscopy is a widely used imaging technique for imaging the three-dimensional distribution of a fluorescence signal in fixed tissue or small organisms. In laser sheet microscopy, the stripe artifacts caused by high absorption or high scattering structures are very common, greatly affecting image quality. To solve this problem, we report here a two-step procedure which consists of continuously acquiring laser sheet images while vertically displacing the sample, and then using the variational stationary noise remover (VSNR) method to further reduce the remaining stripes. Images from a cleared murine colon acquired with a vertical scan are compared with common stitching procedures demonstrating that vertically scanned light sheet microscopy greatly improves the performance of current light sheet microscopy approaches without the need for complex changes to the imaging setup and allows imaging of elongated samples, extending the field of view in the vertical direction.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Di Dong ; Alicia Arranz ; Shouping Zhu ; Yujie Yang ; Liangliang Shi, et al.
"Vertically scanned laser sheet microscopy", J. Biomed. Opt. 19(10), 106001 (Oct 01, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.10.106001


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