Research Papers: Therapeutic

Different doses of low-level laser irradiation modulate the in vitro response of osteoblast-like cells

[+] Author Affiliations
Serena Incerti Parenti, Luigi Checchi

University of Bologna, Department of Biomedical and Neuromotor Sciences, Unit of Periodontology and Orthodontics, via San Vitale 59, 40125 Bologna, Italy

Milena Fini, Matilde Tschon

Rizzoli Orthopaedic Institute, Laboratory of Preclinical and Surgical Studies, via di Barbiano 1/10, 40136 Bologna, Italy

Rizzoli Orthopaedic Institute, Laboratory of Biocompatibility, Technological Innovations and Advanced Therapies, Research, Innovation and Technology Department, via di Barbiano 1/10, 40136 Bologna, Italy

J. Biomed. Opt. 19(10), 108002 (Oct 03, 2014). doi:10.1117/1.JBO.19.10.108002
History: Received July 3, 2014; Revised September 5, 2014; Accepted September 8, 2014
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Abstract.  Because osteoblasts play a key role in bone remodeling and the influence of low-level laser therapy on this process is not clear, Saos-2 human osteoblast-like cells were irradiated by a gallium–aluminum–arsenide diode laser (915 nm) for 10, 48, 96, 193, and 482 s using doses 1, 5, 10, 20, and 50J/cm2, respectively. A control group was not irradiated. Morphology, viability, and cytotoxicity analyses were carried out after 1 hr, 1 day, and 3 days. Deoxyribose nucleic acid (DNA) content and release of vascular endothelial growth factor (VEGF), receptor activator of nuclear factor kappa B ligand (RANKL), and osteoprotegerin (OPG) were evaluated. Viability was modulated by laser irradiation in a dose-dependent manner, with 10J/cm2 inducing a biostimulatory response and 20 to 50J/cm2 determining a bioinhibitory and cytotoxic effect. Accordingly, DNA content was generally increased for the 10J/cm2 dose and decreased for the 50J/cm2 dose. A rapid and transitory trend toward increased RANKL/OPG ratio and a tendency toward a delayed increase in VEGF release for doses of 1 to 10J/cm2 was found. Further investigations using the biostimulatory dose of 10J/cm2 emerged from this study are needed to establish the ideal treatment regimens in the laboratory as well as in clinical practice.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Serena Incerti Parenti ; Luigi Checchi ; Milena Fini and Matilde Tschon
"Different doses of low-level laser irradiation modulate the in vitro response of osteoblast-like cells", J. Biomed. Opt. 19(10), 108002 (Oct 03, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.10.108002


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