Research Papers: Therapeutic

Transcranial low-level laser therapy enhances learning, memory, and neuroprogenitor cells after traumatic brain injury in mice

[+] Author Affiliations
Weijun Xuan

Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Department of Otolaryngology, Nanning 530021, China

Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts 02114, United States

Harvard Medical School, Department of Dermatology, Boston, Massachusetts 02115, United States

Fatma Vatansever

Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts 02114, United States

Harvard Medical School, Department of Dermatology, Boston, Massachusetts 02115, United States

Liyi Huang

Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts 02114, United States

Harvard Medical School, Department of Dermatology, Boston, Massachusetts 02115, United States

Guangxi Medical University, First Affiliated College and Hospital, Department of Infectious Diseases, Nanning 530021, China

Michael R. Hamblin

Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts 02114, United States

Harvard Medical School, Department of Dermatology, Boston, Massachusetts 02115, United States

Harvard-MIT Division of Health Sciences and Technology, Cambridge, Massachusetts 02139, United States

J. Biomed. Opt. 19(10), 108003 (Oct 07, 2014). doi:10.1117/1.JBO.19.10.108003
History: Received August 4, 2014; Revised September 12, 2014; Accepted September 15, 2014
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Abstract.  The use of transcranial low-level laser (light) therapy (tLLLT) to treat stroke and traumatic brain injury (TBI) is attracting increasing attention. We previously showed that LLLT using an 810-nm laser 4 h after controlled cortical impact (CCI)-TBI in mice could significantly improve the neurological severity score, decrease lesion volume, and reduce Fluoro-Jade staining for degenerating neurons. We obtained some evidence for neurogenesis in the region of the lesion. We now tested the hypothesis that tLLLT can improve performance on the Morris water maze (MWM, learning, and memory) and increase neurogenesis in the hippocampus and subventricular zone (SVZ) after CCI-TBI in mice. One and (to a greater extent) three daily laser treatments commencing 4-h post-TBI improved neurological performance as measured by wire grip and motion test especially at 3 and 4 weeks post-TBI. Improvements in visible and hidden platform latency and probe tests in MWM were seen at 4 weeks. Caspase-3 expression was lower in the lesion region at 4 days post-TBI. Double-stained BrdU-NeuN (neuroprogenitor cells) was increased in the dentate gyrus and SVZ. Increases in double-cortin (DCX) and TUJ-1 were also seen. Our study results suggest that tLLLT may improve TBI both by reducing cell death in the lesion and by stimulating neurogenesis.

© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Weijun Xuan ; Fatma Vatansever ; Liyi Huang and Michael R. Hamblin
"Transcranial low-level laser therapy enhances learning, memory, and neuroprogenitor cells after traumatic brain injury in mice", J. Biomed. Opt. 19(10), 108003 (Oct 07, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.10.108003


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