Research Papers: Imaging

Evaluation of transdermal delivery of nanoemulsions in ex vivo porcine skin using two-photon microscopy and confocal laser-scanning microscopy

[+] Author Affiliations
Sanghoon Choi, Yong Joong Lee, Changhwan Kim, Ho Lee

Kyungpook National University, College of Engineering, School of Mechanical Engineering, 80 Daehak-ro Buk-gu, Daegu 702-701, Republic of Korea

Jin Woong Kim

Hanyang University, Department of Applied Chemistry, 55 Hanyangdaehak-ro, Sangnok-gu, Ansan 426-791, Republic of Korea

Hanyang University, Department of Bionano Technology, 55 Hanyangdaehak-ro, Sangnok-gu, Ansan, 426-791, Republic of Korea

Thomas Delmas

Capsum SAS, Heliopolis Batiment C, 3 allée des Maraichers, 13013 Marseille, France

Soyeun Park

Keimyung University, College of Pharmacy, 1095 Dalgubeol-daero Dalseo-gu, Daegu 704-701, Republic of Korea

J. Biomed. Opt. 19(10), 106006 (Oct 09, 2014). doi:10.1117/1.JBO.19.10.106006
History: Received July 25, 2014; Revised September 11, 2014; Accepted September 11, 2014
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Abstract.  This study experimentally evaluates the self-targeting ability of asiaticoside-loaded nanoemulsions compared with nontargeted nanoemulsions in ex vivo experiments with porcine skin samples. Homebuilt two-photon and confocal laser-scanning microscopes were employed to noninvasively examine the transdermal delivery of two distinct nanoemulsions. Prior to the application of nanoemulsions, we noninvasively observed the morphology of porcine skin using two-photon microscopy. We have successfully visualized the distributions of the targeted and nontargeted nanoemulsions absorbed into the porcine skin samples. Asiaticoside-loaded nanoemulsions showed an improved ex vivo transdermal delivery through the stratum corneum compared with nonloaded nanoemulsions. As a secondary measure, nanoemulsions-applied samples were sliced in the depth direction with a surgical knife in order to obtain the complete depth-direction distribution profile of Nile red fluorescence. XZ images demonstrated that asiaticoside-loaded nanoemulsion penetrated deeper into the skin compared with nontargeted nanoemulsions. The basal layer boundary is clearly visible in the case of the asiaticoside-loaded skin sample. These results reaffirm the feasibility of using self-targeting ligands to improve permeation through the skin barrier for cosmetics and topical drug applications.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Sanghoon Choi ; Jin Woong Kim ; Yong Joong Lee ; Thomas Delmas ; Changhwan Kim, et al.
"Evaluation of transdermal delivery of nanoemulsions in ex vivo porcine skin using two-photon microscopy and confocal laser-scanning microscopy", J. Biomed. Opt. 19(10), 106006 (Oct 09, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.10.106006


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