Research Papers: General

Rapid, low-cost fluorescent assay of β-lactamase-derived antibiotic resistance and related antibiotic susceptibility

[+] Author Affiliations
S. Sibel Erdem, Shazia Khan, Akilan Palanisami, Tayyaba Hasan

Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, United States

J. Biomed. Opt. 19(10), 105007 (Oct 10, 2014). doi:10.1117/1.JBO.19.10.105007
History: Received April 29, 2014; Revised June 25, 2014; Accepted July 3, 2014
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Abstract.  Antibiotic resistance (AR) is increasingly prevalent in low and middle income countries (LMICs), but the extent of the problem is poorly understood. This lack of knowledge is a critical deficiency, leaving local health authorities essentially blind to AR outbreaks and crippling their ability to provide effective treatment guidelines. The crux of the problem is the lack of microbiology laboratory capacity available in LMICs. To address this unmet need, we demonstrate a rapid and simple test of β-lactamase resistance (the most common form of AR) that uses a modified β-lactam structure decorated with two fluorophores quenched due to their close proximity. When the β-lactam core is cleaved by β-lactamase, the fluorophores dequench, allowing assay speeds of 20 min to be obtained with a simple, streamlined protocol. Furthermore, by testing in competition with antibiotics, the β-lactamase-associated antibiotic susceptibility can also be extracted. This assay can be easily implemented into standard lab work flows to provide near real-time information of β-lactamase resistance, both for epidemiological purposes as well as individualized patient care.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

S. Sibel Erdem ; Shazia Khan ; Akilan Palanisami and Tayyaba Hasan
"Rapid, low-cost fluorescent assay of β-lactamase-derived antibiotic resistance and related antibiotic susceptibility", J. Biomed. Opt. 19(10), 105007 (Oct 10, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.10.105007


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