Research Papers: Imaging

Spatial versus temporal laser speckle contrast analyses in the presence of static optical scatterers

[+] Author Affiliations
Julio C. Ramirez-San-Juan

Instituto Nacional de Astrofisica, Optica y Electronica, Departamento de Optica, Luis Enrique Erro No. 1, Tonantzintla, Puebla 72840, Mexico

University of California, Irvine, Beckman Laser Institute and Medical Clinic, Department of Surgery, 1002 Health Sciences Road East, Irvine, California 92612, United States

Caitlin Regan

University of California, Irvine, Beckman Laser Institute and Medical Clinic, Department of Surgery, 1002 Health Sciences Road East, Irvine, California 92612, United States

University of California, Irvine, Department of Biomedical Engineering, 3120 Natural Sciences II, Irvine, California 92697, United States

Beatriz Coyotl-Ocelotl

Instituto Nacional de Astrofisica, Optica y Electronica, Departamento de Optica, Luis Enrique Erro No. 1, Tonantzintla, Puebla 72840, Mexico

Bernard Choi

University of California, Irvine, Beckman Laser Institute and Medical Clinic, Department of Surgery, 1002 Health Sciences Road East, Irvine, California 92612, United States

University of California, Irvine, Department of Biomedical Engineering, 3120 Natural Sciences II, Irvine, California 92697, United States

University of California, Irvine, Edwards Lifesciences Center for Advanced Cardiovascular Technology, 2400 Engineering Hall, Irvine, California 92697, United States

J. Biomed. Opt. 19(10), 106009 (Oct 21, 2014). doi:10.1117/1.JBO.19.10.106009
History: Received July 21, 2014; Revised September 19, 2014; Accepted September 22, 2014
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Abstract.  Previously published data demonstrate that the temporal processing algorithm for laser speckle contrast imaging (LSCI) can improve the visibility of deep blood vessels and is less susceptible to static speckle artifacts when compared with the spatial algorithm. To the best of our knowledge, the extent to which the temporal algorithm can accurately predict the speckle contrast associated with flow in deep blood vessels has not been quantified. Here, we employed two phantom systems and imaging setups (epi-illumination and transillumination) to study the contrast predicted by the spatial and temporal algorithms in subsurface capillary tubes as a function of the camera exposure time and the actual flow speed. Our data with both imaging setups suggest that the contrast predicted by the temporal algorithm, and therefore the relative flow speed, is nearly independent of the degree of static optical scattering that contributes to the overall measured speckle pattern. Collectively, these results strongly suggest the potential of temporal LSCI at a single-exposure time to assess accurately the changes in blood flow even in the presence of substantial static optical scattering.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Julio C. Ramirez-San-Juan ; Caitlin Regan ; Beatriz Coyotl-Ocelotl and Bernard Choi
"Spatial versus temporal laser speckle contrast analyses in the presence of static optical scatterers", J. Biomed. Opt. 19(10), 106009 (Oct 21, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.10.106009


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