To quantify changes in SC lumen size in response to changes in pressure within its lumen, the same limbal location was imaged at different pressures: 10, 20, 30, 40, and 50 mm Hg. Pressures were controlled by altering the heights of the reservoir attached to the cannula inserted into SC lumen. The canal area was measured at 10 equally spaced locations (i.e., every ) along a 1 mm length of the limbal segment. The margins of SC lumen were manually delineated and the area then quantified using FIJI (ImageJ software). Figures 5(a), 5(b), and 5(c) show representative OCT images acquired from a single location in the canal at pressures of 10, 30, and 50 mm Hg, respectively. A progressive pressure-dependent increase in dilation of the canal lumen is clearly observed in these images. Figure 5(d) provides a statistical analysis of the area measurements along the canal in this segment. When compared to that of the 10 mm Hg baseline pressure, the mean SC area was increased by at 20 mm Hg, at 30 mm Hg, at 40 mm Hg, and at 50 mm Hg. Statistical analysis of the canal area was made using one-way ANOVA and Tukey paired comparison tests. Significant differences in the canal area were found when comparing (1) 10 and 30 mm Hg (), (2) 10 and 40 mm Hg (), (3) 10 and 50 mm Hg (), and (4) 20 and 50 mm Hg (). The obvious change in lumen size between 10 and 30 mm Hg contrasted with little change between 30 and 50 mm Hg. Our future studies will focus on assessment of pressures between 10 and 30 mm Hg.