About quantum dots (QDs) solution is injected into the shallow hole on the arc surface. The agar-gel without covering is placed in the system and took multiperspective multispectral imaging. We then use the 2-, 4-, and 6-mm slices covering on the arc surface and taking multiperspective multispectral imaging using the system, respectively. The results are processed by multispectral unmixing and target spot center determination using the method mentioned above. Without covering, the spot center coordinates calculated from two perspectives are (212.8184, 236.4625), (291.7266, 236.4625). The coordinates are (212.5323, 236.7462), (291.4989, 236.7462); (212.2375, 237.0362), (291.4989, 237. 0362); (211.6789, 237.5191), (290.9237, 237.5191), when 2-, 4-, and 6-mm slices cover on the arc surface, respectively. Using the reprojection matrix to calculate the four groups of data, the 3-D coordinates under the left camera coordinates system are ; ; ; , respectively. As can be seen, with the increase of slice’s thickness, the accuracy of the data has a certain reduction but very slightly. This experiment simulates the in-vivo fluorescent target at different depths.