Special Section on Laser Applications in Life Sciences

Characterization of mixtures of compounds produced in chlorpromazine aqueous solutions by ultraviolet laser irradiation: their applications in antimicrobial assays

[+] Author Affiliations
Tatiana Alexandru

National Institute for Laser, Plasma and Radiation Physics, Laser Department, Magurele, Ilfov, 077125, Romania

University of Bucharest, Faculty of Physics, Magurele, Ilfov, 077125, Romania

Angela Staicu

National Institute for Laser, Plasma and Radiation Physics, Laser Department, Magurele, Ilfov, 077125, Romania

Alexandru Pascu

National Institute for Laser, Plasma and Radiation Physics, Laser Department, Magurele, Ilfov, 077125, Romania

Elena Radu

National Institute for Research and Development in Chemistry and Petrochemistry, Bucharest, 060021, Romania

Alexandru Stoicu

National Institute for Laser, Plasma and Radiation Physics, Laser Department, Magurele, Ilfov, 077125, Romania

University of Bucharest, Faculty of Chemistry, Bucharest, 030018, Romania

Viorel Nastasa

National Institute for Laser, Plasma and Radiation Physics, Laser Department, Magurele, Ilfov, 077125, Romania

Andra Dinache

National Institute for Laser, Plasma and Radiation Physics, Laser Department, Magurele, Ilfov, 077125, Romania

Mihai Boni

National Institute for Laser, Plasma and Radiation Physics, Laser Department, Magurele, Ilfov, 077125, Romania

University of Bucharest, Faculty of Physics, Magurele, Ilfov, 077125, Romania

Leonard Amaral

New University of Lisbon, Unit of Medical Microbiology, Institute of Hygiene and Tropical Medicine, Group of Mycobacteriology, Lisbon, 1349-008 Portugal

New University of Lisbon, Center from Malaria and Other Tropical Diseases, Institute of Hygiene and Tropical Medicine, Lisbon, 1349-008 Portugal

Mihail Lucian Pascu

National Institute for Laser, Plasma and Radiation Physics, Laser Department, Magurele, Ilfov, 077125, Romania

University of Bucharest, Faculty of Physics, Magurele, Ilfov, 077125, Romania

J. Biomed. Opt. 20(5), 051002 (Nov 03, 2014). doi:10.1117/1.JBO.20.5.051002
History: Received June 2, 2014; Revised July 28, 2014; Accepted August 12, 2014
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Abstract.  The study reports an investigation of the photoproducts obtained by exposure of chlorpromazine hydrochloride in ultrapure water (concentration 2mg/mL) to a 266-nm laser beam obtained by fourth harmonic generation from a Nd:YAG laser (6-ns full time width at half maximum, 10-Hz pulse repetition rate). The photoproducts were analyzed by steady-state UV-Vis absorption, laser-induced fluorescence, Fourier transform infrared spectroscopy, and liquid chromatography–tandem time-of-flight mass spectroscopy. Two figures showing pathways that take place during irradiation for obtaining the final products are shown. The quantum yield of singlet oxygen generation by chlorpromazine (CPZ) was determined relative to standard Zn-phthalocyanine in dimethyl sulfoxide. To outline the role of fluorescence in photoproducts formation rates, fluorescence quantum yield of CPZ during exposure to 355-nm radiation (third harmonic of the fundamental beam of Nd:YAG laser) was investigated relative to standard Coumarin 1 in ethanol. The CPZ solutions exposed 60 and 240 min to 266-nm laser beam, respectively, were tested against Staphylococcus aureus ATCC 25923 strain. For 25μL of CPZ samples irradiated 240 min, a higher diameter of inhibition has obtained against the tested strain than for the 60-min exposed ones.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Tatiana Alexandru ; Angela Staicu ; Alexandru Pascu ; Elena Radu ; Alexandru Stoicu, et al.
"Characterization of mixtures of compounds produced in chlorpromazine aqueous solutions by ultraviolet laser irradiation: their applications in antimicrobial assays", J. Biomed. Opt. 20(5), 051002 (Nov 03, 2014). ; http://dx.doi.org/10.1117/1.JBO.20.5.051002


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