Research Papers: Imaging

Dynamic fluorescence lifetime imaging based on acousto-optic deflectors

[+] Author Affiliations
Wei Yan

Shenzhen University, College of Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen 518060, China

Xiao Peng

Shenzhen University, College of Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen 518060, China

Jing Qi

Shenzhen University, College of Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen 518060, China

Jian Gao

Shenzhen University, College of Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen 518060, China

Shunping Fan

Shenzhen University, College of Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen 518060, China

Qi Wang

Shenzhen University, College of Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen 518060, China

Junle Qu

Shenzhen University, College of Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen 518060, China

Hanben Niu

Shenzhen University, College of Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen 518060, China

J. Biomed. Opt. 19(11), 116004 (Nov 06, 2014). doi:10.1117/1.JBO.19.11.116004
History: Received August 19, 2014; Revised September 25, 2014; Accepted October 13, 2014
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Abstract.  We report a dynamic fluorescence lifetime imaging (D-FLIM) system that is based on a pair of acousto-optic deflectors for the random regions of interest (ROI) study in the sample. The two-dimensional acousto-optic deflector devices are used to rapidly scan the femtosecond excitation laser beam across the sample, providing specific random access to the ROI. Our experimental results using standard fluorescent dyes in live cancer cells demonstrate that the D-FLIM system can dynamically monitor the changing process of the microenvironment in the ROI in live biological samples.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Wei Yan ; Xiao Peng ; Jing Qi ; Jian Gao ; Shunping Fan, et al.
"Dynamic fluorescence lifetime imaging based on acousto-optic deflectors", J. Biomed. Opt. 19(11), 116004 (Nov 06, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.11.116004


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