Special Section on Laser Applications in Life Sciences

Gold nanoaggregates for probing single-living cell based on surface-enhanced Raman spectroscopy

[+] Author Affiliations
Peng Lu

Fujian Normal University, Ministry of Education and Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, Fuzhou 350007, China

Jing Wang

Fujian Normal University, Ministry of Education and Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, Fuzhou 350007, China

Jinyong Lin

Fujian Normal University, Ministry of Education and Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, Fuzhou 350007, China

Juqiang Lin

Fujian Normal University, Ministry of Education and Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, Fuzhou 350007, China

Nenrong Liu

Fujian Normal University, Ministry of Education and Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, Fuzhou 350007, China

Zufang Huang

Fujian Normal University, Ministry of Education and Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, Fuzhou 350007, China

Buhong Li

Fujian Normal University, Ministry of Education and Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, Fuzhou 350007, China

Haishan Zeng

British Columbia Cancer Research Centre, Imaging Unit-Integrative Oncology Department, Vancouver, British Columbia, V5Z 1L3 Canada

Rong Chen

Fujian Normal University, Ministry of Education and Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, Fuzhou 350007, China

J. Biomed. Opt. 20(5), 051005 (Nov 11, 2014). doi:10.1117/1.JBO.20.5.051005
History: Received June 30, 2014; Accepted October 21, 2014
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Abstract.  Gold nanoparticles are delivered into living cells by transient electroporation method to obtain intracellular surface-enhanced Raman spectroscopy (SERS). The subcellular localization of gold nanoparticles is characterized by transmission electron microscopy, and the forming large gold nanoaggregates are mostly found in the cytoplasm. The SERS detection of cells indicates that this kind of gold nanostructures induces a high signal enhancement of cellular chemical compositions, in addition to less cellular toxicity than that of silver nanoparticles. These results demonstrate that rapid incorporation of gold nanoparticles by electroporation into cells has great potential applications in the studies of cell biology and biomedicine.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Peng Lu ; Jing Wang ; Jinyong Lin ; Juqiang Lin ; Nenrong Liu, et al.
"Gold nanoaggregates for probing single-living cell based on surface-enhanced Raman spectroscopy", J. Biomed. Opt. 20(5), 051005 (Nov 11, 2014). ; http://dx.doi.org/10.1117/1.JBO.20.5.051005


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